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pH-ePPE

Plasmid

#183097

Purpose

For plant prime editing in rice plants

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

Insert

Nls-nCas9-NC-nls-MLV-Nls

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRT043-CLYBL-DDdcas9-VPH

Plasmid

#158091

Purpose

Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

Depositor

Martin Kampmann

Article

Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

Insert

CLYBL-CAG-DDdCas9VPH-T2A-GFP

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hSynI-V5-TurboID(N)-GPI

Plasmid

#163696

Purpose

Expresses n-terminal half of TurboID on surface of neurons

Depositor

Scott Soderling

Article

Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

Insert

GPI anchored TurboID

Use

AAV

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3G-LSD1-dCas9-P2A-mCherry

Plasmid

#138462

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

LSD1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mTurquoise2

Plasmid

#98886

Purpose

Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

Plasmid

#107942

Purpose

Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)

Tags

3x HA and NLS (nucleoplasmin)

Expression

Mammalian

Mutation

E174R, S542R, K548R, and N282A

Promoter

CAG

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pIRE4-Azi

Plasmid

#105829

Purpose

Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

Depositor

Irene Coin

Article

Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

Inserts

Humanized EAziRS
4 copies of the U6-BstYam tRNA cassette

Expression

Mammalian

Mutation

(Y37L, D182S, F183M, L186A, D265R)TyrRS

Promoter

PGK and U6

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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PPRE-H2B-eGFP

Plasmid

#84393

Purpose

Fluorescent reporter for PPARgamma activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Insert

PPARG response element (PPARG Human)

Tags

eGFP

Expression

Mammalian

Promoter

PPRE

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

Plasmid

#102245

Purpose

Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

Depositor

Jacob Corn

Insert

dCas9-VPR-mCherry

Use

CRISPR and Lentiviral

Tags

NLS-VPR-mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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3xnls-mScarlet-I

Plasmid

#98816

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pUbC-nls-ha-stdMCP-stdGFP

Plasmid

#98916

Purpose

synonymized tandem dimer MCP fused to synonymized tandem dimer GFP

Depositor

Robert Singer

Article

Wu et al Genes Dev. 2015 Apr 15;29(8):876-86. doi

Insert

stdMCP-stdGFP

Use

Lentiviral

Tags

GFP

Promoter

UbC

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-CMV-VC3AI

Plasmid

#78907

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

VC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-IDH1-R132H

Plasmid

#62907

Purpose

Expresses R132 mutant IDH1 in mammalian cells

Depositor

Yue Xiong

Article

Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

Insert

IDH1 (IDH1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deleted Arg132 (R132)

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDonor_mU6

Plasmid

#69350

Purpose

sgRNA scaffold and mouse U6 promoter

Depositor

Andrea Ventura

Article

Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

Insert

mU6 promoter and sgRNA scaffold flanked by BbsI sites

Use

CRISPR

Promoter

mU6 promoter

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ153

Plasmid

#69304

Purpose

Gateway entry vector with pco-dCas9-3X(SRDX)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pco-dCas9-3X(SRDX) (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

SRDX (X3) repressor fusion; NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Article

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag C/EBPa

Plasmid

#66978

Purpose

Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP alpha (Cebpa Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-IRES

Plasmid

#64784

Purpose

Luciferase reporter vector

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Type

Empty backbone

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-ETV2-IRES-Puro

Plasmid

#61061

Purpose

lentiviral vector for ETV2

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

EF1alpha

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAR4

Plasmid

#61619

Purpose

FRET-based biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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