We narrowed to 10,106 results for: HA
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Plasmid#145624PurposeBacterial Expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046851
Plasmid#145614PurposeBacterial Expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046737
Plasmid#145605PurposeBacterial Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046817
Plasmid#145607PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCuHA-SpAtg101(414)
Plasmid#122081PurposeExpressing S. pombe HA-Atg101DepositorInsertSpATG101
UseTags3HAExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
KHT-1
Plasmid#113054PurposeExpresses worm KHT-1 in mammalian cellsDepositorInsertK+ channel for Habituation to Tap subunit 1
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 N4A 3xFLAG
Plasmid#111975PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.DepositorInsertHsh155 N4A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationT178A R181A R182A R186APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K818A 3xFLAG
Plasmid#111982PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. LethalDepositorInsertHsh155 K818A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationK818APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R775A 3xFLAG
Plasmid#111977PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. LethalDepositorInsertHsh155 R775A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR775APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R778A 3xFLAG
Plasmid#111979PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. LethalDepositorInsertHsh155 R778A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR778APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiN HPV8 E6 K142A
Plasmid#87680PurposeLentiviral expression of HPV8 E6 K142ADepositorInsertHPV 8E6
UseLentiviralTagsFlag and HAExpressionMutationK142APromoterCMVAvailable sinceMarch 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiN MmuPV1 E6 R130A
Plasmid#87679PurposeLentiviral expression of MmuPV1 E6 R130ADepositorInsertMmuPV1 E6
UseLentiviralTagsFlag and HAExpressionMutationR130APromoterCMVAvailable sinceMarch 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_GB
Plasmid#78677PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_FB
Plasmid#78676PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_EB
Plasmid#78675PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_AB
Plasmid#78674PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS2
Plasmid#65668PurposeMYC expression vector with RBPMS2DepositorInsertRBPMS2 (RBPMS2 Human)
UseTagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB786
Plasmid#60151PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET:CACT:GFP
Plasmid#60737PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
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p6671 MSCV-IP N-HAonly 38E6
Plasmid#37056DepositorInsertHPV38 E6
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHWBF06EC
Plasmid#130827PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseGateway entry vectorTagsmCherry-FLAG-HAExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
SUN2delSUNec
Plasmid#130847PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseGateway entry vectorTagsmCherry-FLAG-HAExpressionMutationDeletion of SUN domainPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
SUN2delCCec
Plasmid#130848PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
UseGateway entry vectorTagsmCherry-FLAG-HAExpressionMutationDeletion of coiled coil domainPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE
Plasmid#131730PurposeMADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strainDepositorInsertTagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsTagBFP2-V5 tagExpressionMammalianMutationG12VPromoternone (4x polyA to mitigate episomal expression)Available sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic PancACe
Plasmid#215708PurposeExpresses acetyl-CoA biosensor in the cytoplasmDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial PancACe
Plasmid#215710PurposeExpresses acetyl-CoA biosensor in the mitochondriaDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mitochondrial cpGFP
Plasmid#215711PurposeExpresses cpGFP in the mitochondriaDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and mitochondrial localization sequence…ExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic cpGFP
Plasmid#215709PurposeExpresses cpGFP in the cytoplasmDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-APOBEC1-YTH
Plasmid#178949PurposeLentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cellsDepositorInsertAPOBEC1-YTH-T2A-GFP
UseLentiviral and Synthetic Biology; InducibleTagsHAExpressionMammalianMutationPromotertight TREAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-CDC25A
Plasmid#216511PurposeExpresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoterDepositorInsertCDC25A (CDC25A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
mito NAD+ biosensor
Plasmid#186791PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondriaDepositorInsertmito NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 wt
Plasmid#80876Purposemammalian expression of ALK5 WTDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
Plasmid#60910PurposedCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag systemDepositorInsertdCas9
UseCRISPR and LentiviralTags2x NLS, HA, and NLSExpressionMammalianMutationPromoterdSV40 PromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only