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Addgene

We narrowed to 10,106 results for: HA

Showing: 7841 - 7880 of 10106 results
  1. pGBW-m4046771

    Plasmid
    #145624
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. pGBW-m4046851

    Plasmid
    #145614
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 helicase
    Insert
    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. pGBW-m4046737

    Plasmid
    #145605
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pGBW-m4046817

    Plasmid
    #145607
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. Hsh155 N4A 3xFLAG

    Plasmid
    #111975
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.
    Depositor
    Insert
    Hsh155 N4A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    T178A R181A R182A R186A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. Hsh155 K818A 3xFLAG

    Plasmid
    #111982
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. Lethal
    Depositor
    Insert
    Hsh155 K818A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    K818A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. Hsh155 R775A 3xFLAG

    Plasmid
    #111977
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. Lethal
    Depositor
    Insert
    Hsh155 R775A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    R775A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. Hsh155 R778A 3xFLAG

    Plasmid
    #111979
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. Lethal
    Depositor
    Insert
    Hsh155 R778A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    R778A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. 36INS2-pBAD_GB

    Plasmid
    #78677
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]
    Depositor
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. 36INS2-pBAD_FB

    Plasmid
    #78676
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]
    Depositor
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  11. 36INS2-pBAD_EB

    Plasmid
    #78675
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]
    Depositor
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. 36INS2-pBAD_AB

    Plasmid
    #78674
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]
    Depositor
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. pNB786

    Plasmid
    #60151
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.
    Depositor
    Insert
    FLuc-yEVenus
    Use
    Tags
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
  14. pET:CACT:GFP

    Plasmid
    #60737
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.
    Depositor
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
  15. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid
    #131730
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain
    Depositor
    Insert
    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    TagBFP2-V5 tag
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. mito NAD+ biosensor

    Plasmid
    #186791
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria
    Depositor
    Insert
    mito NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 7841 - 7880 of 10106 results