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pGEX-KG-mTmod3-1-325_domain

Plasmid

#70756

Purpose

mouse Tropomodulin3, 1-325 domain for bacterial expression

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 1-325 AA fragment (Tmod3 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dgcr8_3

Plasmid

#73527

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_4

Plasmid

#73528

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Dgcr8_2

Plasmid

#73526

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

DGCR8

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pCI-MOR30-1

Plasmid

#22336

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR30-1 (Olfr569 Mouse)

Use

Tags

Rho

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)

Plasmid

#60245

Purpose

Express the RBD of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains aa7-192 of Msi1

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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Dendra2-LC-Myosin-N-7

Plasmid

#57729

Purpose

Localization: Light Chain Myosin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Insert

LC-Myosin

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pONL-N1_zyxin

Plasmid

#65683

Purpose

Expresses Zyxin-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

zyxin (Zyx Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2015

Availability

Academic Institutions and Nonprofits only
jk65

Plasmid

#87019

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

SNAP-Nlg1AB
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Dronpa-SSTR3-N-17

Plasmid

#57296

Purpose

Localization: Cilium, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Insert

SSTR3

Use

Tags

Dronpa

Expression

Mammalian

Mutation

A270V in SSTR3

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET-22HT-MSI1(7-192)R61E

Plasmid

#60353

Purpose

R61E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R61E; contains amino acids 7-192 of Msi1

Promoter

T7

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E/R59E/R61E

Plasmid

#60355

Purpose

R53E R59E R61E triple mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E, R59E and R61E mutations; contains amino aci…

Promoter

T7

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pMB84 (Nf2 C-term)

Plasmid

#12169

Depositor

Tyler Jacks

Article

McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Nf2 C-terminal half (Nf2 Mouse)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

C-terminal half of Nf2 cDNA

Promoter

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only
Jam4.1-Fc-His

Plasmid

#72081

Purpose

Expresses the extracellular region of the JAM-4, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam4.1 (Igsf5 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse-MycNV419D-IRES-RFP

Plasmid

#73574

Purpose

Mammalian expression of mouse MycN V419D mutant

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

Mycn (Mycn Mouse)

Use

Retroviral

Tags

IRES-RFP

Expression

Mammalian

Mutation

V419D

Promoter

MSCV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pBD-0032

Plasmid

#12357

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP R362A (Vcp Mouse)

Use

Tags

His6

Expression

Bacterial

Mutation

R362A

Promoter

Available since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
Neo1.c-AP-His

Plasmid

#71965

Purpose

Expresses the extracellular region of the Neogenin 1, isoform c protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.c (Neo1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Plxnb2(L)-AP-His

Plasmid

#72002

Purpose

Expresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb2 (Plxnb2 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-Flag-MyoD(QQQ)-E12

Plasmid

#26003

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Expression

Mutation

Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

Promoter

Available since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector

Plasmid

#73702

Purpose

lentiviral expression of mRIPK3 mutant

Depositor

Francis Chan

Insert

RIPK3 (Ripk3 Mouse)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Mutation

448-AAAA-451

Promoter

CMV/tet

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only
pLIA CRX EnR (CC#145)

Plasmid

#15155

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

CRX (Crx Mouse)

Use

Retroviral

Tags

Engrailed Repressor

Expression

Mammalian

Mutation

amino acids 7–108, containing the CRX homeobox

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pISH-Msx2

Plasmid

#84314

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Msx2 (Msx2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pShuttle AMPK gamma1 H150R

Plasmid

#15999

Depositor

Morris Birnbaum

Insert

AMPK gamma1 (Prkag1 Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Mutation

H150R

Promoter

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO GFP DNAJB10

Plasmid

#19507

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB10 (DNAJB2 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Topo Btg1

Plasmid

#45865

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

Btg1 in situ probe (Btg1 Mouse)

Use

In situ

Tags

Expression

Mutation

contains bp# 1004-1638 of BC006834.1

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-KG-MmUbc6/2790

Plasmid

#11491

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc6 (Ube2j2 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

n/a

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
mEmerald-Talin-Rod

Plasmid

#54270

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Talin-Rod

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Talin Rod Domain w/ K673N (variant CAA39588.1)

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pENTR-CrkII

Plasmid

#50024

Purpose

pENTR vector for mouse Crk-II

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

Crk-II (Crk Mouse)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
pBD-0005

Plasmid

#12281

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP K315L (Vcp Mouse)

Use

Tags

His6

Expression

Bacterial

Mutation

K315L

Promoter

Available since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
pUC57-sgDedd-1

Plasmid

#74435

Purpose

sgRNA expression vector for mouse Dedd gene targeting intron 5

Depositor

Petra Arck

Insert

Dedd (Dedd Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
pGB1-mCRT(P-domain)

Plasmid

#83519

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Use

Tags

GB1 and His

Expression

Bacterial

Mutation

P domain (contains residues 187-283)

Promoter

T7

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pGB1-mCRT(C-domain)

Plasmid

#83518

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Immunol. 2016 May 1;196(9):3896-909. d

Insert

CALR (Calr Mouse)

Use

Tags

GB1 and His

Expression

Bacterial

Mutation

C domain (contains residues 340-399)

Promoter

T7

Available since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pBD-0030

Plasmid

#12368

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP R638A (Vcp Mouse)

Use

Tags

His6

Expression

Bacterial

Mutation

R638A

Promoter

Available since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
PBB-79

Plasmid

#8813

Depositor

Cynthia Wolberger

Article

Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

Insert

GABPb1 (1-157) (Gabpb1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
Mouse c-myc (exons II and III)

Plasmid

#8448

Depositor

Frederic Mushinski

Article

Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

Insert

Mouse c-myc (exons II and III) (Myc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Exons II and III only

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
Plxnc1-AP-His

Plasmid

#72005

Purpose

Expresses the extracellular region of the PlexinC1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnc1 (Plxnc1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBD-0028

Plasmid

#12365

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP D598N (Vcp Mouse)

Use

Tags

His6

Expression

Bacterial

Mutation

D598N

Promoter

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only
pInducer21 Flag-Sesn3

Plasmid

#61866

Purpose

Dox inducible lentivirial expression of Flag-Sesn3 (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn3 (Sesn3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
Unc5c.z-Fc-His

Plasmid

#72182

Purpose

Expresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.z (Unc5c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
VCDstub-GFP

Plasmid

#33122

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains a signal sequence fused to a very small …

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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