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KBB464

Plasmid

#185097

Purpose

HA-tagged POM152 with glycosylation sites mutated and with GFP at C-terminus

Depositor

Ken Belanger

Article

Brown et al Biol Open. 2021 Oct 15;10(10). pii: 2722

Insert

POM152

Tags

GFP

Expression

Yeast

Mutation

Pom152DGlyc-GFP

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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LDB34

Plasmid

#185431

Purpose

Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation at amino acid 1041 from the C-term of …

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-hmKO2

Plasmid

#182733

Purpose

Transient expression of humanized mKO2 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

hmKO2

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-hKO1

Plasmid

#182731

Purpose

Transient expression of humanized KO1 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

hKO1

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-mKO1

Plasmid

#182723

Purpose

Transient expression of mKO1 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

mKO1

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-mKO2

Plasmid

#182724

Purpose

Transient expression of mKO2 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

mKO2

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-KO1

Plasmid

#182722

Purpose

Transient expression of KO1 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

KO1

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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p35S-hmKO1

Plasmid

#182732

Purpose

Transient expression of humanized mKO1 in plant cell

Depositor

Yutaka Kodama

Article

Fujii et al Biotechniques. 2018 Apr;64(4):153-161. d

Insert

hmKO1

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-HsTNRC6C_1208-1690-del1419-1510_L

Plasmid

#146882

Purpose

Mammalian Expression of HsTNRC6C_1208-1690-del1419-1510

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6C_1208-1690-del1419-1510 (TNRC6C Human)

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HCV-Fluc_AC

Plasmid

#148447

Purpose

Mammalian Expression of Fluc

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

Fluc

Expression

Mammalian

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a Hyperstabilized 5'SS

Plasmid

#100988

Purpose

pUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGT

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

5'ss mut ATG/GTATGT -> AAG/GTAAGT

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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RP51A-NoBS-NoPseudoBS

Plasmid

#100986

Purpose

RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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lacI-Ptac-lacO-IMBB4-pUC

Plasmid

#170770

Purpose

test plasmid for 5RM assembly, expresses lacI repressor

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Insert

lacI

Use

Synthetic Biology

Promoter

lacI promoter

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMbd3EosHaloTAF_Hyg

Plasmid

#140958

Purpose

Targeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusion

Depositor

Brian Hendrich

Insert

methyl-CpG binding protein 3 (Mbd3 Mouse, Synthetic)

Use

Mouse Targeting

Tags

3xFLAG, Avi, Eos, Halo, and TEV

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJH123

Plasmid

#172146

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.B.(SD4)CD

Expression

Bacterial

Mutation

PylBCD operon (RBS inserted for PylC)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzO139

Plasmid

#172970

Purpose

pWSK29 with Vibrio cholerae wzzO139 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzz gene from Vibrio cholerae O139

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-luc-miR-124

Plasmid

#171558

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-124

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-124

Expression

Mammalian

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD563 CAG MCS (TUPV3)

Plasmid

#139223

Purpose

mMoClo TUPV3, with CAG promoter and an MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPD569 CAG MCS (TUPV9)

Plasmid

#139229

Purpose

mMoClo TUPV9, with CAG promoter and an MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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