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pNM3727

Plasmid

#154115

Purpose

lexO 5X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

lexO 5X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3726

Plasmid

#154114

Purpose

QUAS 5X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

QUAS 5X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2077.2

Plasmid

#140666

Purpose

Controller plasmid for TS circuit and cL and cR controls

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Controller plasmid for TS circuit and cL and cR controls

Use

Synthetic Biology

Expression

Bacterial

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1809_pmax-RBM38-FRB

Plasmid

#118642

Purpose

Transient transfection; Expresses RBM38-FRB; CAGGS promoter

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBM38-FRB

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-FKBP(M)x4

Plasmid

#85003

Purpose

Expresses EGFP-FKBP(M)x4 in yeast

Depositor

Benjamin Glick

Publication

Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

Insert

EGFP-FKBP(M)x4

Expression

Yeast

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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E2-Crimson-FKBP(L)

Plasmid

#85001

Purpose

Expresses E2-Crimson-FKBP(L)

Depositor

Benjamin Glick

Publication

Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

Insert

E2-Crimson-FKBP(L)

Expression

Yeast

Available since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRAV12

Plasmid

#67713

Purpose

Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Publication

Kieft et al RNA. 2004 Jun;10(6):988-95.

Insert

Transcription cassette for 3'-tagged RNAs

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 RNA polymerase

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTac-E-luci

Plasmid

#65937

Purpose

Theophylline Riboswitch E with luciferase gene

Depositor

Justin Gallivan

Publication

Mishler et al Nucleic Acids Res. 2014 Jun;42(10):6753-

Insert

luciferase

Expression

Bacterial

Available since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
pAW-GFP-2xmiR-34 (pAW-AS-25)

Plasmid

#40833

Purpose

miRNA sensor used in Drosophila cell lines

Depositor

Phillip Zamore

Publication

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

Two target sites perfectly complementary to miR-34

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pPB_TRE3G_scFV-sfGFP-Kmt5cFL

Plasmid

#235581

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3

Depositor

Jamie Hackett

Publication

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Kmt5c (Kmt5c Mouse)

Use

CRISPR

Available since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

Plasmid

#218666

Purpose

sgRNA targeting human EIF2AK3/PERK

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK3 (EIF2AK3 Human)

Use

CRISPR and Lentiviral

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTagBFP2-WPRE

Plasmid

#193339

Purpose

AAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Publication

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mTagBFP2

Use

AAV

Expression

Mammalian

Mutation

A XhoI site in the coding sequence is silenced

Promoter

TRE

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-EGFP MASTL WT (siRNA resistant)

Plasmid

#191011

Purpose

Expresses EGFP-tagged MASTL WT with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

Depositor

Johanna Ivaska

Publication

Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Microtubule-associated serine/threonine kinase like (MASTL Human)

Tags

EGFP

Expression

Mammalian

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMT85_PS-mEos3.2_GentaR

Plasmid

#173895

Purpose

Transposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoter

Depositor

Yonathan Arfi

Publication

Rideau et al Microbiol Spectr. 2022 Jun 29;10(3):e006

Insert

mEos3.2

Expression

Bacterial

Promoter

Spiralin

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)

Plasmid

#149278

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Publication

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pORTMAGE312B

Plasmid

#128969

Purpose

Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

Depositor

George Church

Publication

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E32K

Expression

Bacterial

Mutation

E32K

Promoter

xylS-Pm

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.13/AUG-FFLuc-3XFLAG

Plasmid

#127333

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CV40

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

Plasmid

#127312

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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ESR1-luciferase

Plasmid

#113351

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Luciferase

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6015nes

Plasmid

#108657

Purpose

A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Publication

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-S6K-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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