We narrowed to 9,083 results for: mel

1. pEF1a-2xSV40_NLS-NLuc

   Plasmid

   #135953

   Purpose

   Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Tags

   2xSV40-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV FLEX Synaptophysin GFP

   Plasmid

   #137188

   Purpose

   Cre-dependent expression of synaptophysin-GFP

   Depositor

   Matthew Nolan

   Article

   Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

   Insert

   Synaptophysin GFP (Syp Mouse)
   

   Use

   AAV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

   Plasmid

   #177850

   Purpose

   Bacterial Expression of AMPK

   Depositor

   Karsten Melcher

   Article

   Yan et al Science. 2021 Jul 23;373(6553):413-419.

   Insert

   AMPK (a1b2g1)
   

   Tags

   MBP

   Expression

   Bacterial

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

   Plasmid

   #115516

   Purpose

   Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

   Depositor

   Marco Morsch

   Article

   Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

   Insert

   MLS-KillerRed
   

   Use

   Gateway cloning

   Tags

   MLS

   Promoter

   UAS

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFLAG AXL

   Plasmid

   #105933

   Purpose

   Mammalian expression of Flag-tagged AXL

   Depositor

   Rosa Marina Melillo

   Article

   Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

   Insert

   AXL (AXL Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINDUCER20_mStrawberry

   Plasmid

   #161734

   Purpose

   Doxycycline-inducible expression of mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Expression

   Mammalian

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. delCMV-mCherry-2x-Rhotekin-GBD

   Plasmid

   #214142

   Purpose

   Rho activity sensor

   Depositor

   Leif Dehmelt

   Article

   Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

   Insert

   3xRhotekin-GBD (Rtkn Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Only the GTPase binding domain (GBD) is included

   Promoter

   delCMV

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

   Plasmid

   #184635

   Purpose

   Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gat1 (Slc6a1 Mouse)
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MiniCoopR U6:gRNA spred1, mitfa:Cas9

   Plasmid

   #118843

   Purpose

   Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   spred1 gRNA (spred1 Zebrafish)
   

   Use

   CRISPR; Tol2

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-USPL1-pcDNA3.1

    Plasmid

    #85760

    Purpose

    mammalian expression of full length HA tagged USPL1

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length (USPL1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28b-hUba2-His

    Plasmid

    #53117

    Purpose

    expression of full length human Uba2 with Cterminal His-tag in E.coli

    Depositor

    Frauke Melchior

    Article

    Werner et al Methods Mol Biol. 2009;497:187-99. doi:

    Insert

    UBA2 (UBA2 Human)
    

    Tags

    His and T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-cT3G-cERP2-PRDM1

    Plasmid

    #222924

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of PRDM1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    PRDM1 (PRDM1 Human)
    

    Expression

    Mammalian

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28C-mCherry-DDX4LCD

    Plasmid

    #204408

    Purpose

    E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    DDX4(1-236) (DDX4 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28C-mCherry-53BP1LCD

    Plasmid

    #204407

    Purpose

    E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    53BP1(1208-1977) (TP53BP1 Human)
    

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. "AND-gate" receiver plasmid

    Plasmid

    #127676

    Purpose

    Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

    Insert

    LuxR, GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLlacO-1 for LuxR, pLux for GFP

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-cT3G-cERH-ZNF281

    Plasmid

    #222923

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    ZNF281 (ZNF281 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAd MafA-I-nGFP

    Plasmid

    #19412

    Depositor

    Douglas Melton

    Article

    Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

    Insert

    v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa Mouse)
    

    Use

    Adenoviral; Entry vector

    Tags

    GFP and IRES

    Available Since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pHDR2_DAZL-T2A-mGreenLantern-PuroTK

    Plasmid

    #222917

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET23a-His-hRanGAP1tail

    Plasmid

    #53139

    Purpose

    E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    RanGAP1 (RANGAP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    C-terminal fragment (amino acids 398-587)

    Promoter

    T7

    Available Since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. MiniCoopR U6:gRNA p53, mitfa:Cas9

    Plasmid

    #118841

    Purpose

    Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    tp53 gRNA (tp53 Zebrafish)
    

    Use

    CRISPR; Tol2

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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