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pPB-cT3G-cERP2-FOXL2

Plasmid

#192914

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2 (FOXL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAR014 Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

Plasmid

#195548

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-VPR-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 D614G

Plasmid

#164437

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAR028 Lenti pEF-dEnAsCas12a-NLS-ZNF705F KRAB-3XHA-T2A-BSD

Plasmid

#195545

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-TopKRAB-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCROPseq-VEX-optimized-scaffold

Plasmid

#203321

Purpose

Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MYC

Plasmid

#192899

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of MYC

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

MYC (MYC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-FOS

Plasmid

#192924

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of FOS

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOS (FOS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

Plasmid

#187337

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZBTB16

Plasmid

#192903

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ZBTB16

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ZBTB16 (ZBTB16 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAR045 Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD

Plasmid

#195549

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-QNZF-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

Plasmid

#210710

Purpose

This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/N580A

Promoter

M11

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ATF4

Plasmid

#192911

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ATF4

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ATF4 (ATF4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAR012 Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD

Plasmid

#195550

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-ZNF10 KRAB-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

Plasmid

#195547

Purpose

dCas12a effector fusion for manipulating transcription

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

Use

CRISPR and Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-MAFF

Plasmid

#192917

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of MAFF

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

MAFF (MAFF Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-EMX2

Plasmid

#192898

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of EMX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

EMX2 (EMX2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcGFP1-N1-Dsup-deltaC

Plasmid

#90023

Purpose

To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Deletion of C-terminal region

Promoter

CMV

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-JUN

Plasmid

#192895

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of JUN

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

JUN (JUN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a HsDHFR-bio-10His

Plasmid

#193883

Purpose

Expresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequence

Depositor

James Inglese

Article

Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

Insert

human dihydrofolate reductase (DHFR) (DHFR Human)

Use

Tags

His tag and biotinylation sequence

Expression

Bacterial

Mutation

Codon optimized

Promoter

T7

Available since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJT350 Lenti pEF-dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast

Plasmid

#187347

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-MBDa-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge