We narrowed to 9,400 results for: Coli

1. pETDuet-PcPT-ximD-ximE

   Plasmid

   #172654

   Purpose

   Production of decursinol in E. coli

   Depositor

   Minjuan Xu

   Article

   Bu et al ACS Synth Biol. 2020 Sep 18;9(9):2282-22

   Insert

   PcPT, XimD and XimE
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFLIP38

   Plasmid

   #65616

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-edCit-attR1 and attR2-edCer-cMyc

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-pnGFP-Ultra

   Plasmid

   #157923

   Purpose

   Peroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)

   Depositor

   Huiwang Ai

   Article

   Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

   Insert

   pnGFP-Ultra, a high performance peroxynitrite sensor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL80016

   Plasmid

   #50332

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS no stop codon, beta-glucuronidase (E. coli) with two introns
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFLIP43

   Plasmid

   #65618

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Use

   Tags

   6His-edAFPt9-attR1 and attR2-edCer-cMyc

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE80_T5_P3-ehaA_LacI

   Plasmid

   #186315

   Purpose

   Plasmid for inducible E coli expression of the P3 helixCAM.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Insert

   P3-ehaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE80_T5_AP4-ehaA_LacI

   Plasmid

   #186316

   Purpose

   Plasmid for inducible E coli expression of the AP4 helixCAM.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Insert

   AP4-ehaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIA1408

   Plasmid

   #172405

   Purpose

   Expression of SARS-COV-2 Nsp12 D760A in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   D760A

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Plas-CRISPR_reporter

   Plasmid

   #157658

   Purpose

   CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

   Depositor

   Frank Albert

   Article

   Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

   Insert

   dCAS9-VP64, mCherry, KanMX
   

   Use

   Insert storage (replicative in e. coli)

   Tags

   Expression

   Mutation

   N28D in mCherry- please see depositor comments be…

   Promoter

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pReg-ROTU4

    Plasmid

    #190669

    Purpose

    Expresses an evolved rotundine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    ROTU4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGfOMT1-GEN4

    Plasmid

    #190612

    Purpose

    Expresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    GfOMT-GEN4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pReg-GLAU4

    Plasmid

    #190667

    Purpose

    Expresses an evolved glaucine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    GLAU4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pReg-PAP4

    Plasmid

    #190668

    Purpose

    Expresses an evolved papaverine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    PAP4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pReg-NOS4

    Plasmid

    #190670

    Purpose

    Expresses an evolved noscapine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    NOS4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVY-3 sfGFP-tag3

    Plasmid

    #194618

    Purpose

    fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

    Insert

    sfGFP-K(GGSKKRKKR)3
    

    Use

    Tags

    KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFLIP37

    Plasmid

    #65505

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-Cit-attR1 and attR2-Cer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28b-MseLig1

    Plasmid

    #107071

    Purpose

    Expresses M. sedula DNA Ligase 1 in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    DNA ligase 1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-G2K

    Plasmid

    #70169

    Purpose

    bacterial expression of G2K (d-glycerate 2-kinase)

    Depositor

    Paul Sims, George Reed

    Article

    et al Journal of Molecular Catalysis B: Enzyma

    Insert

    garK/G2K
    

    Use

    Tags

    6xHIS and V5

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMH-HT_RcCHAD

    Plasmid

    #127782

    Purpose

    Expresses RcCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    RcCHAD
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAtLCYbSK

    Plasmid

    #53588

    Purpose

    Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

    Insert

    lcyB (LYC Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MS2-ecDHFR

    Plasmid

    #65859

    Purpose

    Expresses RNA-binding protein MS2 with eDHFR fusion tag

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

    Inserts

    MS2
    E. coli Dihydrofolate Reductase (folA )
    

    Use

    Tags

    HA and NLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pCDW115

    Plasmid

    #70062

    Purpose

    artificial transcription template "pause ablation"

    Depositor

    Jeff Gelles

    Article

    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

    Insert

    lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pGEX-4T1 mouse Tankyrase1 5ARC (171–961)

    Plasmid

    #132638

    Purpose

    expresses mouse Tankyrase1 5ARC (171–961) in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Protein Sci. 2018 Jun;27(6):1057-1067. d

    Insert

    Tankyrase1 (171–961)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSETA_crE6

    Plasmid

    #172928

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

    Depositor

    Arnold Boersma

    Article

    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

    Insert

    CrowdingSensorE6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC19-P70a-Bottom-T

    Plasmid

    #87313

    Purpose

    Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Bottom" transcript
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTH07

    Plasmid

    #70063

    Purpose

    artificial transcription template "distal pause"

    Depositor

    Jeff Gelles

    Article

    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

    Insert

    lambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

27. 36INS-J23100_GB

    Plasmid

    #78669

    Purpose

    36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 36INS-J23100_FB

    Plasmid

    #78668

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 36INS-J23100_EB

    Plasmid

    #78667

    Purpose

    36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 36INS-J23100_AB

    Plasmid

    #78666

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated J23100 Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge