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pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

Plasmid

#67984

Purpose

Cas9 activity reporter with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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NPY/Neuropeptide Y scFv [L115/13]

Plasmid

#190505

Purpose

Mammalian Expression of NPY/Neuropeptide Y scFV. Derived from hybridoma L115/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

NPY/Neuropeptide Y (Homo sapiens) recombinant scFV (NPY Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-IRSp53/BAIAP2 [L117/1R]

Plasmid

#177462

Purpose

Mammalian Expression Plasmid of anti-IRSp53/BAIAP2 (Human). Derived from hybridoma L117/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-IRSp53/BAIAP2 (Homo sapiens) recombinant mouse monoclonal antibody (BAIAP2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-ATF4 [N360A/24]

Plasmid

#190315

Purpose

Mammalian Expression Plasmid of anti-ATF4 (Human). Derived from hybridoma N360A/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ATF4 (Homo sapiens) recombinant Mouse monoclonal antibody (ATF4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-PTPR [N165/43R]

Plasmid

#188219

Purpose

Mammalian Expression Plasmid of anti-Pan-PTPR. Derived from hybridoma N165/43

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-PTPR recombinant mouse monoclonal antibody (PTPRF Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PSD-95 scFv [K28/74]

Plasmid

#190490

Purpose

Mammalian Expression of PSD-95 scFV. Derived from hybridoma K28/74.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-95 (Homo sapiens) recombinant scFV (DLG4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

Plasmid

#67990

Purpose

CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

Plasmid

#211969

Purpose

Express gRNA against LIN28A with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting LIN28A (LIN28A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

Plasmid

#211968

Purpose

Express gRNA against LIN28A with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting LIN28A (LIN28A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-SHLD3

Plasmid

#114125

Purpose

Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Genomic integration, tetracycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

Plasmid

#207355

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Bassoon scFv [L124/59]

Plasmid

#182078

Purpose

Mammalian Expression of Bassoon scFv. Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Bassoon (Rattus norvegicus) (Bsn Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-SPHKAP [L131/17R]

Plasmid

#177477

Purpose

Mammalian Expression Plasmid of anti-SPHKAP (Mouse). Derived from hybridoma L131/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SPHKAP (Mus musculus) recombinant mouse monoclonal antibody (Sphkap Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(JARID2_5-4)-PGKpuroBFP-W

Plasmid

#211966

Purpose

Express gRNA against JARID2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting JARID2 (JARID2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-HRAS(G12V)-IRES-p53DD

Plasmid

#233185

Purpose

Retroviral expression of HRAS(G12V) and p53DD (RIDD)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-p53DD (HRAS, Trp53 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

G12V

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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Homer1L scFv [L113/27]

Plasmid

#190502

Purpose

Mammalian Expression of Homer1L scFV. Derived from hybridoma L113/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Homer1L (Mus musculus) recombinant scFV (Homer1 Mouse)

Tags

Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV6-MPP8-10xHis

Plasmid

#194178

Purpose

Expresses MPP8-10xHis tagged

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Nat Commun. 2022 Nov 2;13(1):6563. doi:

Insert

MPHOSPH8 (MPHOSPH8 Human)

Tags

10xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Kir6.1 K+ channel scFv [N366/60]

Plasmid

#190547

Purpose

Mammalian Expression of Kir6.1 K+ channel scFV. Derived from hybridoma N366/60.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj8 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpM6 [N470/22R]

Plasmid

#206596

Purpose

Mammalian Expression Plasmid of anti-TrpM6 (Human). Derived from hybridoma N470/22.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpM6 (Homo sapiens) recombinant Mouse monoclonal antibody (TRPM6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cav1.2 Ca2+ channel scFv [N263/31]

Plasmid

#182083

Purpose

Mammalian Expression of Cav1.2 Ca2+ channel scFv. Derived from hybridoma N263/31.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Cav1.2 Ca2+ channel (Rattus norvegicus) (Cacna1c Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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