We narrowed to 14,039 results for: TIM

1. CRY2(535, L348F)-mCherry

   Plasmid

   #75372

   Purpose

   Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 residues 1-535 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   L348F

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-FOXO3a TM-ER

   Plasmid

   #8353

   Depositor

   Michael Greenberg

   Article

   Tran et al Science 2002 Apr 19;296(5567):530-4.

   Insert

   FOXO3a (FOXO3 Human)
   

   Tags

   HA (YDVPDYASL)

   Expression

   Mammalian

   Mutation

   T32A, S253A, S315A, mouse estrogen receptor (G525…

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

3. CRY2PHR(W349R)-mCherry

   Plasmid

   #75370

   Purpose

   Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 residues 1-498 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   W349R

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

   Plasmid

   #50942

   Purpose

   Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

   Depositor

   Tobias Bonhoeffer, Mark Huebener, Tobias Rose

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6s
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-FLEX-GT

   Plasmid

   #26198

   Depositor

   Edward Callaway

   Article

   Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   Enhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)
   

   Use

   AAV and Cre/Lox

   Mutation

   TVA mammalian codon optimized

   Available Since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEGFP-HRas S17N

   Plasmid

   #18665

   Depositor

   Karel Svoboda

   Article

   Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

   Insert

   H-Ras (HRAS Human)
   

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   S17N mutation in H-Ras

   Available Since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. Gal4BD-CRY2(535)

   Plasmid

   #75369

   Purpose

   Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Tags

   Gal4BD

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPS0381

   Plasmid

   #133231

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPRv2_mSTING_scrambled_gRNA_2

   Plasmid

   #196628

   Purpose

   Scrambled control 2 for STING knock-out in murine cells.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING scrambled gRNA 2
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-Tet-Puro-shIAPEz_2

    Plasmid

    #185017

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_IAPEz
    

    Use

    Lentiviral

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-Tet-Puro-shIAPEz_1

    Plasmid

    #185016

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_IAPEz
    

    Use

    Lentiviral

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

    Plasmid

    #169328

    Purpose

    Expression of HaloTag9 located at the microtubules of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CEP41-HaloTag9 (CEP41 Human)
    

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAB1679 CMV-HIVNES-GS-Cas13bt3

    Plasmid

    #176317

    Purpose

    CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt3
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

    Plasmid

    #50943

    Purpose

    Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

    Depositor

    Tobias Bonhoeffer, Mark Huebener, Tobias Rose

    Article

    Rose et al Science. 2016 Jun 10;352(6291):1319-22.

    Insert

    mRuby2-P2A-GCaMP6f
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP gRNA (BRDN0000561167)

    Plasmid

    #80034

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR-cBEST-v2-PermE

    Plasmid

    #209446

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PtipA; sgRNA expression from ermE* promoter

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5/FRT/TO_TOMM20_HaloTag9

    Plasmid

    #169335

    Purpose

    Expression of HaloTag9 at the mitochondrial surface of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag9 (TOMM20 Human)
    

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid

    #83239

    Purpose

    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCpdCr
    

    Tags

    roGFP2 fusion with Tsa2dCpdCr

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Twitch-2B pRSETB

    Plasmid

    #48203

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2B
    

    Tags

    6xHIS, cpVenusCD, and mCerulean3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-FOXO3a TM-ER-delta DB

    Plasmid

    #8354

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, ER (G525R), delta DB (deletio…

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only