We narrowed to 7,454 results for: ski

1. pET-51b(+)-Caprola_01

   Plasmid

   #194662

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_01 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_01
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. U6-CjCas9-MS2gRNA-Backbone

   Plasmid

   #210711

   Purpose

   This plasmid express MS2 loop containing Cj Cas9 gRNA

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MS2 loop containing Cj Cas9 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXS-IRES-Blast coSHMT2

   Plasmid

   #106299

   Purpose

   Expresses SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 2 (SHMT2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized

   Promoter

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDH-SIRT2-Flag

   Plasmid

   #102624

   Purpose

   Expresses full length SIRT2 isoform 1 in mammalian cells

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   SIRT2 (SIRT2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP

   Plasmid

   #194696

   Purpose

   HuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafish

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_01-mEGFP
   

   Use

   Zebrafish expression

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   elavl3(HuC)

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-51b(+)-cpHalo∆

   Plasmid

   #194659

   Purpose

   Protein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   cpHalo∆
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

   Plasmid

   #172501

   Purpose

   Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 full locus with Golden gate acceptor site for spacer
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX Flag RagC S75N

   Plasmid

   #112757

   Purpose

   express Flag-RagC (S75N mutant)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-RagC (S75N) (RRAGC Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   S75N

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CathepsinG-CD4d3+4-bio

   Plasmid

   #73125

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding CathepsinG with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   Cathepsin G (CTSG Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEND-193_Cacnes_HS

    Plasmid

    #225622

    Purpose

    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    HspR
    

    Use

    Synthetic Biology

    Tags

    pFAST-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. miniABEMax V82G wt

    Plasmid

    #135362

    Purpose

    Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    miniABEMax nSpyCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUTKan-3xHA-GFP-TOM5

    Plasmid

    #130674

    Purpose

    Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

    Depositor

    Andreas Weber

    Article

    Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

    Insert

    Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
    

    Use

    Tags

    3xHA and sGFP

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin10

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEND-303_Cacnes_tetR

    Plasmid

    #225618

    Purpose

    C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    TetR
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEND-192_Cacnes_HS

    Plasmid

    #225621

    Purpose

    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    HspR
    

    Use

    Synthetic Biology

    Tags

    pFAST-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ10713

    Plasmid

    #183958

    Purpose

    U6-crSox2-CAG-hyperdCas12a-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    crRNA targeting Sox2
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-MBP-TEVpCS-ADGRG6 CTF-FLAG

    Plasmid

    #217704

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGR6 (ADGRG6 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-840

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

    Plasmid

    #101349

    Purpose

    2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta tat delta TAR delta env GFP in Nef Tet ope…

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-MBP-TEVpCS-ADGRL2 CTF-FLAG

    Plasmid

    #217707

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL2 (ADGRL2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-824

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmCherry-MYRIP

    Plasmid

    #89586

    Purpose

    Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYRIP (Myrip Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CaMKII-iGECI

    Plasmid

    #160423

    Purpose

    AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge