165,075 results

1. pLX208 CMV sTurboID (C)-HA-FRB-ERM

   Plasmid

   #153007

   Purpose

   Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID (C) (G74)-ERM (Cb5)
   

   Use

   Lentiviral

   Tags

   FRB and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDB_053

   Plasmid

   #216083

   Purpose

   for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
   

   Use

   Destination

   Expression

   Insect

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-6xGLY-3xFLAG-kanMX6

   Plasmid

   #20754

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Expression

   Yeast

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAMD1-2

   Plasmid

   #61564

   Purpose

   Standardized mini-Tn5 delivery plasmid, kanamycin resistant

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

   Type

   Empty backbone

   Use

   Synthetic Biology; Mini-tn5 vector

   Available Since

   Jan. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPtGE34

   Plasmid

   #107932

   Purpose

   Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

   Depositor

   David Edgell

   Article

   Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

   Inserts

   OriT
   40SRPS8 Promoter
   ShBle
   40SRPS8 Terminator
   Cen6-ArsH4-His3
   

   Use

   CRISPR and Synthetic Biology; Episomal vector for…

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. UbcH5c WT pET28a

   Plasmid

   #12643

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
   

   Expression

   Bacterial

   Available Since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIIS793

   Plasmid

   #238206

   Purpose

   Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   TaTasR
   

   Tags

   TwinStrepII-bdSUMO

   Expression

   Bacterial

   Available Since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-KIR2DL4-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156662

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DL4 (KIR2DL4 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR-PsppA-mCherry

   Plasmid

   #225428

   Purpose

   Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

   Depositor

   Geir Mathiesen

   Article

   Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

   Insert

   mCherry, Cas9, SgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PsppA upstream of mCherry and Cas9, P3 upstream o…

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-KASH2

    Plasmid

    #187017

    Purpose

    Inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    KASH2
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11b-MIF

    Plasmid

    #149446

    Purpose

    Expression of WT MIF in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cx36-GCaMP

    Plasmid

    #123604

    Purpose

    Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

    Depositor

    John O'Brien

    Article

    Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

    Insert

    Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
    

    Tags

    GCaMP3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pm-iATPSnFR1.0

    Plasmid

    #102548

    Purpose

    membrane displayed ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Expression

    Mammalian

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNCS mStrawberry

    Plasmid

    #91768

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Strawberry
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG424-10X-tRNA

    Plasmid

    #70125

    Purpose

    Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

    Depositor

    Jay Hesselberth

    Article

    Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

    Insert

    10X-tRNA block
    

    Expression

    Yeast

    Promoter

    None – SUP4 pol III promoter provided by insert

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pU6-BbsI-chiRNA

    Plasmid

    #45946

    Purpose

    Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

    Depositor

    Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    U6-BbsI-chiRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    Dm-snRNA:U6:96Ab

    Available Since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. ATG9A-mCherry

    Plasmid

    #197393

    Purpose

    Expresses ATG9A in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

    Insert

    ATG9A (ATG9A Human)
    

    Tags

    mCh

    Expression

    Mammalian

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-GfaABC1D-ERCreER-4x6T

    Plasmid

    #196411

    Purpose

    Astrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    ERT2-Cre-ERT2
    

    Use

    AAV; Astrocyte-selective

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSUP-(2xCouRS-tRNAopt)

    Plasmid

    #197930

    Purpose

    Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

    Inserts

    CouRS
    tRNA opt
    

    Expression

    Bacterial

    Promoter

    glnS' and lambda(ts) and proK

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOT_2 - lenti-EFS-tNGFR-2A-puro

    Plasmid

    #181971

    Purpose

    Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    tNGFR (NGFR Human)
    

    Use

    Lentiviral

    Mutation

    truncation: aa 11-276

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPtPBR11

    Plasmid

    #80386

    Purpose

    Episome vector for Phaeodactylum tricornutum (pBR322-based)

    Depositor

    Philip Weyman

    Type

    Empty backbone

    Use

    Synthetic Biology; : episomal vector for phaeodac…

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-Syn-DIO-NaChBac-dTomato

    Plasmid

    #236252

    Purpose

    Cre dependent NaChBac-TdTomato expression

    Depositor

    Janelia Viral Tools

    Article

    Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

    Insert

    NaChBac-TdTomato
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. PNCA-GFP

    Plasmid

    #67789

    Purpose

    Use together with pCMV-intron and VSV-G to package MMLV reporter virus

    Depositor

    Stephen Goff

    Article

    Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

    Insert

    MMLV-LTR-GFP
    

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pCap-101-Hgy

    Plasmid

    #227508

    Purpose

    Capture plasmid with hygromycin resistance and pIJ101 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. CA-Stat3-t2A-mCherry

    Plasmid

    #127540

    Purpose

    Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Stat3 (Stat3 Mouse)
    

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders …

    Promoter

    CMV

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCDB327

    Plasmid

    #113671

    Purpose

    expresses Ulp1_R3 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R3
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDONR221_SLC6A8

    Plasmid

    #131915

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A8 (SLC6A8 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. c-Jun

    Plasmid

    #187902

    Purpose

    Expresses c-Jun in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    c-Jun (JUN Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. CRISPR-StAR4GN (Lenti)

    Plasmid

    #222693

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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