We narrowed to 7,181 results for: RAP

1. Sec61b-HA-mNG21-10

   Plasmid

   #141213

   Purpose

   Membrane Tethered Split Fluorescent Reporter for ER Retragrade

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   Sec61b-HA-mNG21-10
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMGS54 (AID-P2A-Hygromycin)

   Plasmid

   #126583

   Purpose

   An AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminal

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   AID-P2A-HygR
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AA294

   Plasmid

   #215968

   Purpose

   Fragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoter

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U1a_v1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA332

   Plasmid

   #216053

   Purpose

   Fragmid fragment: (C' terminus) 3x NLS sequences

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   SV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGK:HygroR-CMV:mKate2-B_UTR

   Plasmid

   #68480

   Purpose

   Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Hygromycin Resistance
   mKate2-B_UTR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   shRNA sensor cassette "B" added to 3&#x…

   Promoter

   CMV and pGK

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211842

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   Promoter

   6xUAS-35Smini and pUBQ10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-neo

   Plasmid

   #166467

   Purpose

   Empty vector to express overexpression cDNA

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVSL10

   Plasmid

   #201182

   Purpose

   Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable

   Depositor

   Jack Szostak

   Article

   Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.

   Insert

   Bst polymerase large fragment (BF) F710Y/D598A
   

   Use

   Tags

   HRV 3C protease cleavage site and His6

   Expression

   Bacterial

   Mutation

   F710Y D598A

   Promoter

   T5-lacO

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVAX1-BMP7

   Plasmid

   #137910

   Purpose

   Constitutive mammalian overexpression vector for human bone morphogenetic protein 7 cDNA

   Depositor

   Georg Feichtinger

   Article

   Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

   Insert

   BMP7 (BMP7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR

    Plasmid

    #188377

    Purpose

    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMuLE ENTR tdTomato L5-L2

    Plasmid

    #62185

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing tdTomato. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    tdTomato
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLyGo-Ec-8

    Plasmid

    #163136

    Purpose

    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P(rhaB)

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK281 (mCherry2-Hygro)

    Plasmid

    #72797

    Purpose

    C-terminal tagging with mCherry2 using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mCherry2-Hygro
    

    Use

    CRISPR

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR221-NR1H3

    Plasmid

    #79514

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    LXR Alpha (NR1H3 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

    Plasmid

    #219781

    Purpose

    CaST-P2A for HEK cell expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTC-M13-GFP-P2A-CaM-V5-sTN
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

    Plasmid

    #105448

    Purpose

    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Has Service

    AAV9

    Insert

    ChrimsonR-mRuby2-ST
    

    Use

    AAV

    Tags

    mRuby2 and soma targeting motif

    Expression

    Mutation

    K176R in Chrimson

    Promoter

    hSyn

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199363

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-AU1-mTOR-E2419K

    Plasmid

    #19994

    Depositor

    Fuyuhiko Tamanoi

    Article

    Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    mammalian target of rapamycin-E2419K (Mtor Rat)
    

    Use

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    Changed Glutamine 2419 to Lysine

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pH6HTC_ABCE1-Halo

    Plasmid

    #175335

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    ABCE1 (ABCE1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR2.1 GFR alpha 2

    Plasmid

    #119899

    Purpose

    Mouse GFR alpha 2 In situ hybridization probe

    Depositor

    Wenqin Luo, David Ginty

    Article

    Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

    Insert

    GDNF family receptor alpha-2 (Gfra2 Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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