We narrowed to 7,455 results for: trac
-
Plasmid#136335PurposeMammalian expression of human p115-RhoGEF with N-terminal V5DepositorInsertARHGEF1 (ARHGEF1 Human)
ExpressionMammalianAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta PBM + Ezrin
Plasmid#202418PurposeExpression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRINDepositorAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP2A-WT-neo
Plasmid#156177PurposeLentiviral vector for expression of RAP2A-WT, with neomycin selectionDepositorInsertRAP2A (RAP2A Human)
UseLentiviralExpressionMammalianMutationwild type formPromoterhuman PGKAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTBL716 4xHRE-YB-TATA-Cas9-ODD-T2A-TdT
Plasmid#132667PurposeExpresses hypoxia-inducible Cas9 and TdT in mammalian cells.DepositorInsertCas9-ODD-T2A-TdT (DNTT Human, Streptococcus pyogenes)
ExpressionMammalianPromoter4xHRE_YB TATAAvailable SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSbi-pur-EGFP-KSR1-CA3 (N-KSR)
Plasmid#217769PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400PromoterEF-1alpha promoterAvailable SinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLXI_TRC403 LacZ
Plasmid#111184PurposeControl inducible vector with LacZ (V5 tagged)DepositorInsertlacZ
UseLentiviralTagsV5PromoterTREAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLXI_TRC401 LacZ
Plasmid#111183PurposeControl inducible vector with LacZ (V5 tagged)DepositorInsertlacZ
UseLentiviralTagsV5PromoterTREAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
tetO-HEY2
Plasmid#170691Purposedoxycycline-inducible overexpression of HEY2DepositorInsertHEY2 – hes related family bHLH transcription factor with YRPW motif 2 (HEY2 Human)
UseLentiviral; Doxycycline inducibleExpressionMammalianPromoterTRE promoter, Tet-ONAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKSR1-CA3-EGFP (C-KSR)
Plasmid#217753PurposeFluorescent reporter for ceramide (mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterCMVAvailable SinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKSR1-CA3-mRFP1 (C-KSR)
Plasmid#217754PurposeFluorescent reporter for ceramide (mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
TagsmRFPExpressionMammalianMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterCMVAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSbi-pur-KSR1-CA3-EGFP (C-KSR)
Plasmid#217768PurposeFluorescent reporter for ceramide (for stable mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterEF-1alpha promoterAvailable SinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)
Plasmid#217767PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400PromoterT7 promoterAvailable SinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)
Plasmid#217766PurposeFluorescent reporter for ceramide (for bacterial expression and purification)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterT7 promoterAvailable SinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PA-DAD-dark
Plasmid#53096PurposeExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformationDepositorInsertLOV(dark)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in Venus; C450M mutation in LOV2Available SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG
Plasmid#69114PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationL1939A and L1942A amino acid substitutions. Sile…PromoterCMVAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag
Plasmid#210820PurposeMammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)DepositorTagsMyc, 3xFlag and TwinStrep, 10xHisExpressionMammalianPromoterCMV and CMV / IRES2Available SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-WT-neo
Plasmid#156173PurposeLentiviral vector for expression of RAP1A-WT, with neomycin sectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationwild type formPromoterhuman PGKAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-E63-neo
Plasmid#156174PurposeLentiviral vector for expression of RAP1A-E63, with neomycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationchanged Glutamine 63 to Glutamic acidPromoterhuman PGKAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-flag-RAP1B-V12-puro
Plasmid#156168PurposeLentiviral vector for expression of flag-RAP1B-V12, with puromycin selectionDepositorInsertRAP1B (RAP1B Bovine)
UseLentiviralTags2xFLAGExpressionMammalianMutationchanged Glycine 12 to ValinePromoterhuman PGKAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Cas9-T2A-STOP-TdT
Plasmid#126425PurposeExpresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)DepositorInsertCas9-T2A-STOP-TdT (DNTT Human, Streptococcus pyogenes)
ExpressionMammalianMutationalanine-6 of TdT has been changed to a STOP codonPromoterpCMVAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only