3,614 results

1. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

   Plasmid

   #63166

   Purpose

   GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-HA"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

   Plasmid

   #154786

   Purpose

   Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

   Depositor

   Robert Singer

   Article

   Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

   Insert

   Adenosine deaminse (Adar Fly, Synthetic)
   

   Use

   Lentiviral

   Tags

   GFP, V5, and mCherry

   Expression

   Mammalian

   Mutation

   Synonymized serine to prevent autoediting of ADAR…

   Promoter

   UBC

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

   Plasmid

   #154787

   Purpose

   Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

   Depositor

   Robert Singer

   Article

   Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

   Insert

   Adenosine deaminse (Adar Fly, Synthetic)
   

   Use

   Lentiviral

   Tags

   GFP, V5, and stdMCP

   Expression

   Mammalian

   Mutation

   Synonymized serine to prevent autoediting of ADAR…

   Promoter

   UBC

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT-ADARcd-E488Q-V5

   Plasmid

   #139686

   Purpose

   Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

   Depositor

   Michael Rosbash

   Article

   Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

   Insert

   Adenosine deaminase acting on RNA (Adar Fly)
   

   Use

   Tags

   V5

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFD3-dU6:3gRNA

   Plasmid

   #49410

   Purpose

   expression of gRNA under control of the Drosophila U6:3 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-3:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-3

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217515

   Purpose

   Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a_DmKHC[1-421]-1xGFP-6xHis

   Plasmid

   #196972

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. H3K9me3 biosensor

   Plasmid

   #120802

   Purpose

   FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUASP dsRed Rab7 WT

   Plasmid

   #44824

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab7 (Rab7 Fly)
   

   Use

   Drosophila

   Tags

   dsRed

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. KHBD00727

    Plasmid

    #39748

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9932 (CG9932 Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only