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Showing: 21 - 40 of 319 results

  1. pRL488

    Plasmid
    #70263
    Purpose
    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.
    Depositor
    Peter Wolk
    Article
    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.
    Type
    Empty backbone
    Use
    Luciferase; Cyanobacteria expression
    Tags
    luxAB
    Expression
    Mutation
    Promoter
    none
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70263-plasmid-map-sequence-id-130206

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  2. pRL838

    Plasmid
    #70680
    Purpose
    This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.
    Depositor
    Peter Wolk
    Article
    Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70680-plasmid-map-sequence-id-126382

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  4. pRL_yEGFP_hc

    Plasmid
    #98746
    Purpose
    Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Insert
    Yeast-enhanced green fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    synthetic
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98746-plasmid-map-sequence-id-189855

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  5. pRL542

    Plasmid
    #70282
    Purpose
    ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70282-plasmid-map-sequence-id-125718

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  8. pRL1124

    Plasmid
    #70690
    Purpose
    provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Inserts
    avaiM
    eco47iiM
    ecot22iM
    Use
    Bacterial shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70690-plasmid-map-sequence-id-126465

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  12. pRL1075

    Plasmid
    #70688
    Purpose
    Use of a vector with a conditionally lethal gene, sacB, to help transfer transposon mutations to a wild type strain
    Depositor
    Peter Wolk
    Article
    Black et al Mol Microbiol. 1993 Jul;9(1):77-84.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70688-plasmid-map-sequence-id-126455

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  13. pRL1058

    Plasmid
    #70686
    Purpose
    Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.
    Depositor
    Peter Wolk
    Article
    Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88
    Insert
    Transposon Tn5-1058
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70686-plasmid-map-sequence-id-126440

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  16. pRL439

    Plasmid
    #70258
    Purpose
    Source of psbA promoter of Amaranthus hybridus, used as a strong promoter of cyanobacterial genes
    Depositor
    Peter Wolk
    Article
    Elhai et al Gene. 1988 Aug 15;68(1):119-38.
    Insert
    psbA promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70258-plasmid-map-sequence-id-125568

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  17. pRLH-12

    Plasmid
    #12232
    Depositor
    Sung-Hou Kim
    Insert
    pRas189 Leu12 truncated
    Use
    Tags
    Expression
    Bacterial
    Mutation
    truncated with HindIII to 171aa (from 189aa); Gly…
    Promoter
    Available since
    Sept. 18, 2006
    Availability
    Academic Institutions and Nonprofits only

  20. OPRL1-Tango

    Plasmid
    #66463
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    OPRL1 (OPRL1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 319 results