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pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161925

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

Plasmid

#184951

Purpose

Donor for both PiggyBac and Cp36 Recombinase

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

PuroR, P2A, mCherry

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB

Plasmid

#78348

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

Plasmid

#184952

Purpose

Donor for both PiggyBac and Cp36 Recombinase

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

PuroR, P2A, mTagBFP2

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB-Zeo

Plasmid

#78352

Purpose

Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-KRAB

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJT040 lenti MCS pEF mIFP WPRE

Plasmid

#161929

Purpose

Lentiviral expression of mIFP with multiple cloning site upstream the pEF promoter

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

mIFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161926

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

rTetR(SE-G72P)

Use

Lentiviral and Synthetic Biology

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#196545

Purpose

Donor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

Depositor

Lacra Bintu

Article

DelRosso et al Nature. 2023 Apr;616(7956):365-372. doi:

Insert

Surface marker and Citrine reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo

Plasmid

#179439

Purpose

Expresses rTetR-KRAB(human) for gene silencing, integration by Piggybac

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

H2B-mIFP-T2A-rTetR-KRAB

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSL007_PB_pCMV-H2B-mIFP-T2A-rTetR-ratKRAB-zeo

Plasmid

#179438

Purpose

Expresses rTetR-KRAB(rat) for gene silencing, integration by Piggybac

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

H2B-mIFP-T2A-rTetR-KRAB

Expression

Mammalian

Promoter

pCMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-EED-2A-mTurquoise

Plasmid

#225692

Purpose

Lentiviral expression of rTetR(SE) fused to EED and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-EED (EED Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-HP1a-2A-mTurquoise

Plasmid

#225691

Purpose

Lentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

HP1a (CBX5 Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJT309 RapInducible1-ActivatorCloneSite-Citrine

Plasmid

#187321

Purpose

Rapamycin inducible genetic circuit with cloning site for activator domains

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Type

Empty backbone

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT310 AAV-BFPdonor

Plasmid

#202057

Purpose

AAV HDR donor

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-BFPdonor

Use

AAV; Aav packaging plasmid

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo

Plasmid

#179440

Purpose

Expresses rTetR-HDAC4(human) for gene silencing, integration by Piggybac

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

H2B-mIFP-T2A-rTetR-HDAC4

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJT305 Pa01 Lenti pEF-attB LSR GFP

Plasmid

#184948

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#161928

Purpose

Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

Surface marker and Citrine activation reporter

Use

Synthetic Biology

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#161927

Purpose

Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

Surface marker and Citrine repression reporter

Use

Synthetic Biology

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT301 BxBi Lenti pEF-attB LSR GFP

Plasmid

#184944

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

BxBi Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT306 Kp03 Lenti pEF-attB LSR GFP

Plasmid

#184949

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT302 Ec03 Lenti pEF-attB LSR GFP

Plasmid

#184945

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec03 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT303 Ec04 Lenti pEF-attB LSR GFP

Plasmid

#184946

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec04 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT304 Ec07 Lenti pEF-attB LSR GFP

Plasmid

#184947

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec07 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT311 AAV-Rapamycin Inducible-NZF-Citrine

Plasmid

#202060

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NZF-Citrine

Use

AAV; Aav with reporter

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

Plasmid

#198054

Purpose

All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning

Depositor

Lacra Bintu

Article

Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.

Inserts

rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
3xFLAG-insert

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE3G and pEF

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

Plasmid

#179428

Purpose

Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-(nospacer)-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-rTetR(SE)-DNMT3Bcd-2A-mTurquoise

Plasmid

#225693

Purpose

Lentiviral expression of rTetR(SE) fused to the catalytic domain of DNMT3B and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

rTetR-DNMT3B catalytic domain (DNMT3B Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT314 AAV-Rapamycin Inducible-NZ-Citrine

Plasmid

#202063

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-NZ-Citrine

Use

AAV; Aav with reporter

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT315 AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Plasmid

#202064

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-ZNF473KRAB-Citrine

Use

AAV; Aav with reporter

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT365 AAV-Rapamycin Inducible-S3H

Plasmid

#202066

Purpose

Inducible AAV activator

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-Rapamycin Inducible-S3H

Use

AAV; Aav with reporter

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge