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Showing: 41 - 80 of 325 results

  3. pCytERM-LSSmGFP

    Plasmid
    #186540
    Purpose
    Visualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP
    Use
    Tags
    Cytochrome p450 first 29 animo acids (Oryctolagus…
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186540-plasmid-map-sequence-id-368221

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  8. LSSmKate2-Actin-C-18

    Plasmid
    #56209
    Purpose
    Localization: Actin, Excitation: 460, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Actin (ACTB Human)
    Use
    Tags
    LSSmKate2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pRSETb-LssmsfGFP

    Plasmid
    #112941
    Purpose
    Bacterial expression vector for LSS-msfGFP
    Depositor
    Dorus Gadella
    Insert
    LSS-msfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112941-plasmid-map-sequence-id-220205

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  15. pFRLuc-CoV2-delSS

    Plasmid
    #177615
    Purpose
    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. Slippery sequence (SS) deleted (negative control).
    Depositor
    Junjie Guo
    Article
    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11
    Insert
    SARS-CoV-2 FSE
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Slippery sequence deleted
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177615-plasmid-map-sequence-id-350040

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  19. LifeAct-LSS-SGFP2-N1

    Plasmid
    #112938
    Purpose
    Visualization of F-actin
    Depositor
    Dorus Gadella
    Insert
    LifeAct
    Use
    Tags
    LSS-SGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112938-plasmid-map-sequence-id-223583

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  21. mScarlet-I-LSS-SGFP2

    Plasmid
    #112945
    Purpose
    Produces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRET
    Depositor
    Dorus Gadella
    Insert
    LSS-GFP2 mScarlet-I Fusion
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112945-plasmid-map-sequence-id-223563

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  22. CytERM-LSS-SGFP2

    Plasmid
    #112940
    Purpose
    In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)
    Depositor
    Dorus Gadella
    Insert
    CytERM
    Use
    Tags
    LSS-SGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112940-plasmid-map-sequence-id-222554

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  23. mCherry-LSS-SGFP2

    Plasmid
    #112943
    Purpose
    Produces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRET
    Depositor
    Dorus Gadella
    Insert
    LSS-GFP2 mCherry fusion
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112943-plasmid-map-sequence-id-223564

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  24. Gaq-LSS-SGFP2

    Plasmid
    #112951
    Purpose
    Galphaq tagged with LSS-SGFP2
    Depositor
    Dorus Gadella
    Insert
    Galphaq
    Use
    Tags
    LSS-SGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    112951-plasmid-map-sequence-id-221967

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  25. pRSETb-LSS-mEGFP

    Plasmid
    #112942
    Purpose
    Bacterial expression vector for LSS-EGFP
    Depositor
    Dorus Gadella
    Insert
    LSSS-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112942-plasmid-map-sequence-id-220267

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  26. pRSETb-LSS-SGFP2

    Plasmid
    #112939
    Purpose
    Bacterial expression vector for LSS-SGFP2
    Depositor
    Dorus Gadella
    Insert
    LSS-SGFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112939-plasmid-map-sequence-id-220195

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  28. LSS-SGFP2-aTub

    Plasmid
    #112936
    Purpose
    Visualization of microtubules
    Depositor
    Dorus Gadella
    Insert
    alpha tubulin
    Use
    Tags
    LSS-SGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112936-plasmid-map-sequence-id-220599

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  29. pBa-LSS-GFP-LDLR wt

    Plasmid
    #98184
    Purpose
    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.
    Depositor
    Marvin Bentley, Gary Banker
    Article
    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.
    Insert
    LDLR (LDLR Human)
    Use
    Tags
    GFP and LDLR signal sequence, N-term of GFP so GF…
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Chicken Beta Actin
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98184-plasmid-map-sequence-id-193286

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  35. mScarlet-I-T2A-LSS-SGFP2

    Plasmid
    #112946
    Purpose
    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
    Depositor
    Dorus Gadella
    Insert
    mScarlet-I and LSS-SGFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    112946-plasmid-map-sequence-id-223584

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  37. mCherry-T2A-LSS-SGFP2

    Plasmid
    #112944
    Purpose
    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
    Depositor
    Dorus Gadella
    Insert
    mCherry and LSS-SGFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112944-plasmid-map-sequence-id-220241

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  39. pET28a-LSSmGFP-TEV-SAV

    Plasmid
    #191200
    Purpose
    Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP & Streptavidin fusion
    Use
    Tags
    6xHis and Streptavidin core domain, amino acids 1…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191200-plasmid-map-sequence-id-376986

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Showing: 41 - 80 of 325 results