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Plasmid#222587PurposeBB3 gsdA tet-on overexpression cassette with homologous flanks for integration at pyrG locusDepositorArticleInsertgsdA (gsdA )
UseA. nigerTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insert,…Promotertet-onAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSF8
Plasmid#222592PurposeBB2 gsdA tet-on overexpression cassetteDepositorArticleInsertgsdA
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insert,…Promotertet-onAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-RR-zrif1_exon8
Plasmid#194434PurposeRight (RR) TALEN for mutating zebrafish rif1 geneDepositorInsertzrif1_ex8_RIGHT (rif1 Zebrafish)
UseTALENTagsExpressionMutationPromoterCMV IE94Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2TAL3-DD-zrif1_exon8
Plasmid#194435PurposeLeft (DD) TALEN for mutating zebrafish rif1 geneDepositorInsertzrif1_ex8_LEFT (rif1 Zebrafish)
UseTALENTagsExpressionMutationPromoterCMV IE94Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GATA1
Plasmid#194436PurposeIn Situ hybridization probesDepositorInsertGATA1a (gata1a Zebrafish)
UseIn situ hybridizationTagsExpressionMutationPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTT]-nLuc
Plasmid#199757PurposeElongation reporter construct to quantify elongation of synYFP[CTT]DepositorInsertleuYFP[CTT]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-leuYFP[CTC]-nLuc
Plasmid#199758PurposeElongation reporter construct to quantify elongation of synYFP[CTC]DepositorInsertleuYFP[CTC]
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTW072
Plasmid#190247PurposeT-DNA for arabidopsis transformationDepositorInsertgRNA, Cas9, firefly luciferase, bar
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTW069
Plasmid#190246PurposeT-DNA for arabidopsis transformationDepositorInsertgRNA, Cas9, firefly luciferase, bar
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzpHS2
Plasmid#172971PurposepWSK29 with S. flexneri wzzpHS2 geneDepositorInsertwzzPHS2 gene from Shigella flexneri
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzO139
Plasmid#172970PurposepWSK29 with Vibrio cholerae wzzO139 geneDepositorInsertwzz gene from Vibrio cholerae O139
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBSF
Plasmid#172967PurposepWSK29 with S. flexneri 2a wzzBSF geneDepositorInsertwzzB gene from Shigella flexneri
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA E171Q HDEL
Plasmid#32123DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationK108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA K108A HDEL
Plasmid#32121DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationK108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKM197
Plasmid#132665PurposepyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.DepositorInsertUpstream & Downstream pyrE deletion region
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-GGS4X-GFP
Plasmid#108583PurposeExpresses PKAcs-GFP fusion in mammalian cells with flexible linkerDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-nLuc
Plasmid#199756PurposeElongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stallingDepositorInsertsynYFP[TTG/AGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
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PKAcs-GGSD5X-GFP
Plasmid#108584PurposeExpresses PKAcs-GFP fusion in mammalian cells with flexible linkerDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE GFP
Plasmid#32120DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303PromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA K108A GFP
Plasmid#32125DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP, HDEL, and Kar2ExpressionYeastMutationK108APromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-KR6X-GFP
Plasmid#108585PurposeExpresses PKAcs-GFP fusion in mammalian cells with structured linkerDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-L10X-GFP
Plasmid#108587PurposeExpresses PKAcs-GFP fusion in mammalian cells with structured linkerDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOR22-2-ISH
Plasmid#15839DepositorInsertMOR22-2 (Olfr69 Mouse)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE E171Q GFP
Plasmid#32128DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, E171QPromoterAvailable sinceOct. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE HDEL
Plasmid#32118DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationdeletion of E at 302 or 303PromoterAvailable sinceOct. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAG78 3'UTR
Plasmid#12023DepositorInsertN2 3'UTR
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG84 3'UTR
Plasmid#12026DepositorInsertN5 3'UTR
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMOR11-2-ISH
Plasmid#15837DepositorInsertMOR11-2 (Olfr33 Mouse)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc
Plasmid#199759PurposeElongation reporter construct to quantify elongation duration of 4_CGA stallingDepositorInsertsynYFP[TTG/AGA]-4xArg[CGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc
Plasmid#199760PurposeElongation reporter construct to quantify elongation duration of 6_CGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[CGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc
Plasmid#199761PurposeElongation reporter construct to quantify elongation duration of 6_AGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[AGA]-cLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pProbe_CUSPER
Plasmid#185953Purposebroad host plasmid that can be used to construct reproductive success bioreporterDepositorInsertUnspecified
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH-09
Plasmid#158437PurposepBAD33 vector encoding MotA, Dendra2 - GSGSGS linker - MotBDepositorInsertsMotA
MotB
UseTagsDendra2- 3 x EAAAK linkerExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-25
Plasmid#158436PurposepBAD33 vector encoding MotA, YPet - 3 x EAAAK linker - MotBDepositorInsertsMotA
MotB
UseTagsyPET - 3 x EAAAK linkerExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-05
Plasmid#158435PurposepBAD33 vector encoding MotA, YPet - EAAAK linker - MotBDepositorInsertsMotA
MotB
UseTagsyPET - EAAAK linkerExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-nolinker-GFP
Plasmid#108579PurposeExpresses PKAcs-GFP fusion in mammalian cellsDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-G12X-GFP
Plasmid#108588PurposeExpresses PKAcs-GFP fusion in mammalian cells with flexible linkerDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE E171Q HDEL
Plasmid#32124DepositorInsertTorsinA (TOR1A Human)
UseTagsHDEL and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, E171QPromoterAvailable sinceOct. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinADE K108A GFP
Plasmid#32126DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationdeletion of E at 302 or 303, K108APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only