Addgene

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pRK5 FLAG metap2

Plasmid

#32004

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

metap2 (Metap2 )

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pKRG3-mU6-PUb-hSpCas9

Plasmid

#162162

Purpose

Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

N/A

Expression

Insect

Mutation

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-puro human lipin 1-1 shRNA

Plasmid

#32019

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pSL1180-HR-PUbEGFP-NLS

Plasmid

#176681

Purpose

Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom0.6

Plasmid

#87353

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 0.6 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

0.6 kbp proximal promoter of human MCT2 (nested d…

Available since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2-MCT2Prom4.2

Plasmid

#87347

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 4.2 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

4.2 kbp proximal promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-MCT2Prom2.5

Plasmid

#87351

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

2.5 kbp proximal promoter of human MCT2 (nested d…

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-MCT2Prom1.1

Plasmid

#87352

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

1.1 kbp proximal promoter of human MCT2 (nested d…

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2-MCT2Prom3.2

Plasmid

#87349

Purpose

Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp

Depositor

Saroj Mathupala

Article

Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

Insert

MCT2 (SLC16A7 Human)

Use

Luciferase

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

3.2 kbp proximal promoter of MCT2 (nested deletio…

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-hSpCas9-pAc

Plasmid

#162163

Purpose

Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

T2A-pAc

Expression

Insect

Mutation

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSL1180-HR-PUbEBFP2-NLS

Plasmid

#176682

Purpose

Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

EBFP2

Use

Tags

Expression

Insect

Mutation

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKRG3-mU6-PUb-3xFLAG-hSpCas9

Plasmid

#162161

Purpose

Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LZF1827 pAAV_hSyn-Flp-off-Cre-on-stGtACR2-CFP

Plasmid

#135413

Purpose

expresses GtACR2 linked to CFP

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

stGtACR2-CFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2

Plasmid

#135412

Purpose

simultaneous optical recording and manipulation of electrical activity in neurons

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

SomArchon-EGFP-P2A-stGtACR2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
UB061 (FCK-stGtACR2-fusionRed)

Plasmid

#135765

Purpose

expresses soma targeted GtACR2

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

stGtACR2

Use

Lentiviral

Tags

Fusion Red

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG wildtype, catalytic active lipin 1

Plasmid

#32005

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAaePUb::Cas9-2A-Neo

Plasmid

#176680

Purpose

Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)

Use

Crispr

Tags

Expression

Insect

Mutation

Drosophila codon optimized

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized

Plasmid

#172345

Purpose

Expression of FLAG-tagged, codon-optimized, full-length SRSF3

Depositor

Sujatha Jagannathan

Article

Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

Insert

serine and arginine rich splicing factor 3 (SRSF3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized

Promoter

EF-1a

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 FLAG wildtype, catalytic dead lipin 1

Plasmid

#32006

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

D712E and D714E

Promoter

CMV, SP6

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pLKO-puro FLAG wildtype, catalytic active lipin 1

Plasmid

#32010

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only