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pEGFP-N1 hMOG-alpha1-EGFP

Plasmid

#126463

Purpose

Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

Insert

Myelin oligodendrocyte glycoprotein (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Lifeact-EGFP-2XNLS

Plasmid

#58467

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Lifeact
2X NLS with 2X stop codons

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP-3XNLS

Plasmid

#58466

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Utr230 with 3X glycine linker (UTRN Human)
3X NLS with 3X stop codons

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

Plasmid

#162880

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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peGFP(N1)-deltaCMV-rSyntaxin1a-meGFP

Plasmid

#34631

Depositor

Wolfhard Almers

Article

Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

Insert

rat Syntaxin1a (Stx1a Rat)

Use

Tags

meGFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 RPEL1-EGFP-3XNLS

Plasmid

#58469

Purpose

Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

MRTF-A RPEL1 (Mrtfa Mouse, Synthetic)
3X NLS with 3X stop codons

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP mCherry-ApoB-EGFP

Plasmid

#112859

Purpose

Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

mCherry-apoB-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha2-EGFP

Plasmid

#160976

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha2 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha3-EGFP

Plasmid

#160977

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha3 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta2-EGFP

Plasmid

#160979

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta3-EGFP

Plasmid

#160980

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta3 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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EGFR∆550-580-mEGFP

Plasmid

#203788

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

EGFR (EGFR Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

aa 550-580 deleted

Promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-EGFR

Plasmid

#222325

Purpose

Synchronize the trafficking of EGFR from the ER.

Depositor

Franck Perez

Article

Scharaw et al J Cell Biol. 2016 Nov 21;215(4):543-558.

Insert

Streptavidin-KDEL and EGFR fused to SBP-EGFP (EGFR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

Plasmid

#138152

Purpose

Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

eGFP-MCS

Use

Lentiviral

Tags

2PEST AND NLS

Expression

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

Plasmid

#188634

Purpose

HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

EGFR (EGFR Human, Synthetic)

Use

CRISPR

Tags

CD3ε-mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE

Plasmid

#89609

Purpose

over-expression of VEGF-A(165) in human cells

Depositor

Fernando Fierro

Insert

VEGF-A(165) (VEGFA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K188E

Promoter

MNDU3

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-EGFR NLS

Plasmid

#192315

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-EGFR NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-EGFR NLS (EGFR Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-vsv-Mis12-dCEN-Incenp-EGFP

Plasmid

#108478

Purpose

expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

Plasmid

#74513

Purpose

AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

EGFP-p2A-EGFP-f

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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XZ362 EF1a-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

Plasmid

#233381

Purpose

Optimized VEGF-sensing eLIDAR with VEGFR2 ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGFR2(ecto+TM)-tdMCP

Use

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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