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Showing: 21 - 40 of 22169 results

  1. pLH_Scr15

    Plasmid
    #100537
    Purpose
    CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    N-terminal version of PhyB (AA 1-621)/ Mutation o…
    Promoter
    FBA1 and TDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100537-plasmid-map-sequence-id-194456

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  3. pJYScr

    Plasmid
    #132719
    Purpose
    Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance
    Depositor
    Jan Marienhagen
    Article
    Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    132719-plasmid-map-sequence-id-259037

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  4. pLH_Scr16

    Plasmid
    #100538
    Purpose
    CEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Cdc1 NLS, CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    AA 1-621 of PhyB/ Mutation nucleotide 576 C-->A
    Promoter
    FBA1 and TDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100538-plasmid-map-sequence-id-194462

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  8. pGL3_RNF157_SOX9scr_neg0str

    Plasmid
    #232373
    Purpose
    RNF157 enhancer, SOX9 site scrambled, buffering site strengthened
    Depositor
    Joanna Wysocka
    Insert
    RNF157 (RNF157 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    RNF157 enhancer, SOX9 site scrambled, buffering s…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232373-plasmid-map-sequence-id-462873

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  9. pGL3_PTCHD4_pos2pos0pos1scramble

    Plasmid
    #232382
    Purpose
    PTCHD4 enhancer, three sensitizing elements scrambled
    Depositor
    Joanna Wysocka
    Insert
    PTCHD4 (PTCHD4 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    PTCHD4 enhancer, three sensitizing elements scram…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232382-plasmid-map-sequence-id-462860

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  10. pGL3_THADA_allscramble

    Plasmid
    #232386
    Purpose
    THADA enhancer, all sensitizing elements scrambled
    Depositor
    Joanna Wysocka
    Insert
    THADA (THADA Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    THADA enhancer, all sensitizing elements scrambled
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232386-plasmid-map-sequence-id-462861

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  11. pGL3_IMPG1_lpos0scr

    Plasmid
    #232394
    Purpose
    IMPG1 enhancer, one sensitizing element scrambled
    Depositor
    Joanna Wysocka
    Insert
    IMPG1 (IMPG1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    IMPG1 enhancer, one sensitizing element scrambled
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232394-plasmid-map-sequence-id-462854

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  12. pGL3_RNF157_SOX9scramble

    Plasmid
    #232376
    Purpose
    RNF157 enhancer, SOX9 site scrambled
    Depositor
    Joanna Wysocka
    Insert
    RNF157 (RNF157 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    RNF157 enhancer, SOX9 site scrambled
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232376-plasmid-map-sequence-id-462847

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  13. pGL3_MSR1_bothneg0scramble

    Plasmid
    #232383
    Purpose
    MSR1 enhancer, both buffering Coordinators scrambled
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    MSR1 enhancer, both buffering Coordinators scramb…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232383-plasmid-map-sequence-id-462848

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  14. pGL3_RNF157_neg0SOX9scramble

    Plasmid
    #232379
    Purpose
    RNF157 enhancer, SOX9 site and buffering site scrambled
    Depositor
    Joanna Wysocka
    Insert
    RNF157 (RNF157 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    RNF157 enhancer, SOX9 site and buffering site scr…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232379-plasmid-map-sequence-id-462844

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  15. pGL3_IMPG1_lpos0pos1mpos0scr

    Plasmid
    #232396
    Purpose
    IMPG1 enhancer, three sensitizing elements scrambled
    Depositor
    Joanna Wysocka
    Insert
    IMPG1 (IMPG1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    IMPG1 enhancer, three sensitizing elements scramb…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232396-plasmid-map-sequence-id-462842

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  16. pGL3_IMPG1_lpos0pos1scr

    Plasmid
    #232395
    Purpose
    IMPG1 enhancer, two sensitizing elements scrambled
    Depositor
    Joanna Wysocka
    Insert
    IMPG1 (IMPG1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    IMPG1 enhancer, two sensitizing elements scrambled
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232395-plasmid-map-sequence-id-462836

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  17. pSCrhaB2_ApyOAHIDS

    Plasmid
    #226246
    Purpose
    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS
    Depositor
    Wilfred van der Donk
    Article
    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103
    Inserts
    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    Use
    Tags
    Hexa-Histidine
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    226246-plasmid-map-sequence-id-450056

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Showing: 21 - 40 of 22169 results