We narrowed to 450 results for: crispr library

1. MDM4_CRISPRi_gRNA_2

   Plasmid

   #195133

   Purpose

   gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 CRISPRi gRNA 2 (MDM4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. MDM4_CRISPRi_gRNA_1

   Plasmid

   #195132

   Purpose

   gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 CRISPRi gRNA 1 (MDM4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9+sgRNA expression vector precursor

   Plasmid

   #171798

   Purpose

   PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

   Depositor

   Stephen Elledge

   Article

   Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO

   Available Since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiAsCpf1-3xMYC-blast

   Plasmid

   #127750

   Purpose

   Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal

   Depositor

   Giulio Draetta

   Article

   Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

   Insert

   AsCpf1
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDonor_sU6

   Plasmid

   #69351

   Purpose

   sgRNA scaffold and synthetic U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   sU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   synthetic U6 promoter

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDonor_mU6

   Plasmid

   #69350

   Purpose

   sgRNA scaffold and mouse U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   mU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   mU6 promoter

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDonor_hU6

   Plasmid

   #69312

   Purpose

   sgRNA scaffold and human U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   hU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   human U6 promoter

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiUniversal-Puro

   Plasmid

   #127749

   Purpose

   Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)

   Depositor

   Giulio Draetta

   Article

   Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX-sgRNA-BfuAI-2k

   Plasmid

   #112915

   Purpose

   Empty sgRNA expression plasmid

   Depositor

   Ren-Jang Lin

   Article

   Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-dual-scafffold-2.0 (pBP49)

    Plasmid

    #218931

    Purpose

    The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Insert

    dual scaffolds for CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pxpr_502-wt-ltr

    Plasmid

    #182647

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCas9-Blast

    Plasmid

    #52962

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Lentiviral Prep

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJR152

    Plasmid

    #196280

    Purpose

    Cassette used in dual sgRNA library generation

    Depositor

    Rebecca Voorhees

    Article

    Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

    Insert

    hU6-sgRNA-CR3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ampl43

    Plasmid

    #161830

    Purpose

    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-sgRNA-Lib

    Plasmid

    #53121

    Purpose

    sgRNA expression construction in a 3rd generation lentiviral backbone

    Depositor

    Wensheng Wei

    Article

    Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiguide-puro-wt-ltr

    Plasmid

    #182635

    Purpose

    Expresses guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCas9n(D10A)-Blast

    Plasmid

    #63593

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Blasticidin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, nickase mutant

    Promoter

    EFS-NS

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pxpr_050-wt-ltr

    Plasmid

    #182646

    Purpose

    lentiviral expression of gRNA scaffold, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    None
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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