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We narrowed to 5,045 results for: golden gate

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Showing: 21 - 40 of 5045 results

  7. pTU2_2a

    Plasmid
    #73010
    Purpose
    Secondary module (BpiI) site in pTU2a backbone
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Secondary module + Golden Gate pTU2a BsmBI destination site
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    73010-plasmid-map-sequence-id-133861

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  9. pTU2_2b

    Plasmid
    #73011
    Purpose
    Secondary module (BpiI) site in pTU2b backbone
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Secondary module + Golden Gate pTU2b BsmBI destination site
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    73011-plasmid-map-sequence-id-133862

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  12. pTU2S-a (p15A origin)

    Plasmid
    #74088
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 2 TU's with secondary module and p15A origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74088-plasmid-map-sequence-id-138296

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  13. pTU2S-b (p15A origin)

    Plasmid
    #74089
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74089-plasmid-map-sequence-id-138299

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  16. pTU2S-a (colE1 origin)

    Plasmid
    #74091
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74091-plasmid-map-sequence-id-138318

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  17. pTU2S-b (colE1 origin)

    Plasmid
    #74092
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74092-plasmid-map-sequence-id-141928

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  18. cBEST4

    Plasmid
    #234660
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and tcp830
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234660-plasmid-map-sequence-id-472864

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  19. pTU2-A-RFP (p15A origin)

    Plasmid
    #74090
    Purpose
    pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    R0010 Const. Promoter controlling RFP
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74090-plasmid-map-sequence-id-138311

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  20. cBEST2

    Plasmid
    #234658
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    kasO*17tss and kasOp*
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234658-plasmid-map-sequence-id-492748

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Showing: 21 - 40 of 5045 results