We narrowed to 10,302 results for: 158

1. EGFP-UTRN-mini

   Plasmid

   #158749

   Purpose

   Expresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cells

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

   Insert

   UTRN-mini
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-10xPP7 oligo-library based

   Plasmid

   #158199

   Purpose

   Cassette of 10 different binding sites with high affinity to PP7 phage coat protein, as extracted from the oligo pool experiment.

   Depositor

   Roee Amit

   Article

   Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

   Insert

   10 binding sites of PP7 from the oligo library
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. MAC-SARS-CoV-2 Orf6

   Plasmid

   #158374

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 Orf6 (ORF6 )
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pKL12-GCaMP6f-pHuji bb118

   Plasmid

   #158981

   Purpose

   Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   GCaMP6f-pHuji
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#100833 a…

   Promoter

   Bba_J23118 (sigma 70)

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p663-UBC-miniTurbo-V5-DYRK1B_IDG-K

   Plasmid

   #158183

   Purpose

   Gateway destination clone of DYRK1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-DYRK1B (DYRK1B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. KRAS-P34R

   Plasmid

   #158527

   Purpose

   Expresses human KRAS P34R in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

   Depositor

   Kenneth Westover

   Article

   Bera et al Birth Defects Res. 2020 Mar 18. doi: 10.

   Insert

   KRAS (KRAS Human)
   

   Use

   Tags

   6xHis-tag and TEV protease cleavage sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC-SARS-CoV-2 E

   Plasmid

   #158368

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 E (E )
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. MAC-TMPRSS2

   Plasmid

   #158385

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pRGEB34

   Plasmid

   #158152

   Purpose

   Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN_TT 3xFLAG-MAVS

    Plasmid

    #158628

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 601-145-8X

    Plasmid

    #158620

    Purpose

    DNA fragment production for nucleosome core particle preparation

    Depositor

    Curt Davey

    Article

    Vasudevan et al J Mol Biol. 2010 Oct 15;403(1):1-10. doi

    Type

    Empty backbone

    Use

    Bacterial vector for dna production

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CuO-V5 CASC3 siRes 1-480 188/218-mut

    Plasmid

    #158542

    Purpose

    For PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480

    Depositor

    Niels Gehring

    Article

    Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

    Insert

    CASC3; BTZ; MLN51 (CASC3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Amino acids 1-480, C-terminal deletion, F188D, W2…

    Promoter

    CMV

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_S.AU1.VSVg_NGFR

    Plasmid

    #158351

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.AU1.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAC-SARS-CoV-2 NSP1

    Plasmid

    #158352

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP1 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pLEX307-CTSB-G418

    Plasmid

    #158465

    Purpose

    Constitutive lentiviral expression of human CTSB.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    CTSB (CTSB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. GAL FLAG46QDProGFP p306

    Plasmid

    #15832

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 46Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

17. p663-UBC-miniTurbo-V5-CDK18_IDG-K

    Plasmid

    #158180

    Purpose

    Gateway destination clone of CDK18 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK18 (CDK18 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-hSyn-Gαi*-BERKY3

    Plasmid

    #158428

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pISH-Venus

    Plasmid

    #15865

    Depositor

    Peter Mombaerts

    Article

    Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

    Insert

    Venus
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pYPQ-dzCas9-NG-Act3.0

    Plasmid

    #158415

    Purpose

    CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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