We narrowed to 4,313 results for: Pet2

1. pET28a(+)- PC-MjMAT

   Plasmid

   #179372

   Purpose

   PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

   Depositor

   Andrea Rentmeister

   Article

   Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

   Insert

   PC-MjMAT
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L147A, I351A

   Promoter

   T7

   Available Since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28HistevTtTERT(1-516)

   Plasmid

   #28171

   Depositor

   Kathleen Collins

   Article

   O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

   Insert

   Tetrahymena TERT
   

   Tags

   6xhistidine

   Expression

   Bacterial

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-UBC9

   Plasmid

   #228949

   Purpose

   express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET29-neoLux1.2

   Plasmid

   #229952

   Purpose

   Bacterial expression for de novo luciferase, neoLux1.2

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   neoLux1.2
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-RILP

   Plasmid

   #74600

   Purpose

   used for expression in E. coli and purification

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab-interacting lysosomal protein (RILP Human)
   

   Tags

   FLAG, StrepII, and superfolder GFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a MDH_AQUAR

   Plasmid

   #204239

   Purpose

   Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDH_AQUAR
   

   Tags

   6X His

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-cMSP1D1

   Plasmid

   #199768

   Purpose

   Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP1D1
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24a MDH_9CREN

   Plasmid

   #204243

   Purpose

   Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDH_9CREN
   

   Tags

   6X His

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22HT-PP7delFG

   Plasmid

   #31863

   Depositor

   Robert Singer

   Article

   Chao et al Nat Struct Mol Biol. 2008 Jan;15(1):103-

   Insert

   PP7delFG Coat Protein
   

   Tags

   His6-tag and TEV site

   Mutation

   deleted FG loop

   Available Since

   Aug. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-mCherryGGBsMTS

    Plasmid

    #133625

    Purpose

    Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherryGGBsMTS
    

    Expression

    Bacterial

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_Caspase-4_K356D

    Plasmid

    #214312

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    K356D

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-T4uvsW

    Plasmid

    #228201

    Purpose

    Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

    Depositor

    Stephen Benkovic

    Article

    Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

    Insert

    uvsW
    

    Tags

    hexahistidine and 10 amino acid linker

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. NylB'_pET21b

    Plasmid

    #215416

    Purpose

    Expression construct for NylB' gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'
    

    Expression

    Bacterial

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET20b-mPTER

    Plasmid

    #222189

    Purpose

    Bacterial expression of mouse PTER protein

    Depositor

    Jonathan Long

    Article

    Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

    Insert

    PTER (Pter Mouse)
    

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b-NM23-H1

    Plasmid

    #157987

    Purpose

    Expresses NM23-H1 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Han et al Bulletin of the Kor Chem Soc 2010; 31(5)

    Insert

    NM23-H1 (NME1 Human)
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24c(+)-EFTs

    Plasmid

    #31173

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Ts
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

17. GSK3beta/pET29b

    Plasmid

    #16315

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

    Insert

    GSK3beta (gsk3b.L Frog)
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pET28GST-LIC

    Plasmid

    #26101

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6XHis, GST, and Thrombin CLeavage Site

    Expression

    Bacterial

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET28-2H12

    Plasmid

    #221186

    Purpose

    His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29a-hNABP2

    Plasmid

    #128307

    Purpose

    Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y85C- please see depositor comments below

    Promoter

    T7

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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