We narrowed to 8,828 results for: Pol

1. pcDNA-Ran-Q69L-mRFP1-polyA

   Plasmid

   #104560

   Purpose

   Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Q69L

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. single polypeptide FPX biosensor for caspase-3

   Plasmid

   #60883

   Purpose

   FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for caspase-3
   

   Use

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-TgKI-MCS-tdTomato-polyA

   Plasmid

   #174022

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   tdTomato
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR P2R-P3-SV40 polyA signal

   Plasmid

   #48627

   Purpose

   Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   SV40 early polyadenylation signal cassette
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-HA-hTet1CD-WPRE-PolyA

   Plasmid

   #39454

   Depositor

   Hongjun Song

   Article

   Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

   Insert

   HA-hTet1CD (TET1 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa1418–2136

   Promoter

   EF1a

   Available since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pET empty polycistronic destination vector (2D)

   Plasmid

   #29774

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFH2.30_Gag-MCP-dpol-T2A-eGFP

   Plasmid

   #205538

   Purpose

   Express HIV Gag-MCP-dpol-T2A-eGFP

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   HIV Gag-MCP-dpol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   pol frameshift sequence recoded

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

   Plasmid

   #163543

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   POLD2-APOBEC1-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS aP2 promoter (5.4kb) polyA

   Plasmid

   #11424

   Depositor

   Ronald Kahn

   Insert

   aP2 promoter (Fabp4 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. single polypeptide FPX biosensor for caspase-1

    Plasmid

    #60885

    Purpose

    FPX biosensor for caspase-1

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-1
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCDH-EF1-FHC-POLQ-K121M

    Plasmid

    #64877

    Purpose

    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M, a mutation in the conserved ATP-binding si…

    Promoter

    EF1alpha

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-EF1-FHC-POLQ-DY2230AA

    Plasmid

    #64878

    Purpose

    lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    D2330A,Y2331A, in the DNA polymerase domain (POL)…

    Promoter

    EF1alpha

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-45b-POLR2A-CTD-mCherry

    Plasmid

    #185011

    Purpose

    Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    POLR2A (Polr2a Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. single polypeptide FPX biosensor for caspase-8

    Plasmid

    #60884

    Purpose

    FPX biosensor for caspase-8

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-8
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

15. MMLV-CAG-G_oT7pol-IRES-BSD

    Plasmid

    #172369

    Purpose

    Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    oT7pol + BSD
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET empty polycistronic destination vector (2E)

    Plasmid

    #29775

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. mpeg:Rab7-stop-polyA; cmlc2:GFP

    Plasmid

    #213738

    Purpose

    Expresses mCherry-Rab7 in the macrophage lineage of zebrafish with a green heart transgenesis marker

    Depositor

    William Talbot

    Article

    Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

    Insert

    mCherry-Rab7
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    mpeg1.1

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA

    Plasmid

    #39455

    Depositor

    Hongjun Song

    Article

    Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

    Insert

    HA-hTet1CDmu (TET1 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa1418–2136 with mutations H1672 to Y, 1674D to A

    Promoter

    EF1a

    Available since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

19. IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA

    Plasmid

    #121750

    Purpose

    pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    3x HA epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)

    Plasmid

    #156162

    Purpose

    Expresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    THBS1 with deletion of amino acid residues 383-548

    Promoter

    CMV

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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