We narrowed to 26,178 results for: STI;

1. p931-SWITCH-OVER insert: EF1a-Blasti

   Plasmid

   #217890

   Purpose

   insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCA528 SPASTIN , F124D, residues 108-200

   Plasmid

   #180629

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200, F124D

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA528 SPASTIN , L177D, residues 108-200

   Plasmid

   #180634

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200, L177D

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

   Plasmid

   #198765

   Purpose

   expression of hOCT4 mutants

   Depositor

   Hyonchol Jang

   Article

   Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

   Insert

   hOCT4 236D (POU5F1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S236D

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB UbC Arrestin-uTEV1-V5; UAS-mCherry

   Plasmid

   #173122

   Purpose

   Expresses uTEV1 and reporter receiver construct in DIPG

   Depositor

   Alice Ting

   Article

   Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

   Insert

   Arrestin-uTEV1-V5; UAS-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherry

   Plasmid

   #173121

   Purpose

   Expresses TEVp(WT) and reporter receiver construct in DIPG

   Depositor

   Alice Ting

   Article

   Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

   Insert

   Arrestin-TEVp(WT)-V5; UAS-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target

   Plasmid

   #159842

   Purpose

   Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   pDESTR4-R3 mosTI Pmlc-2_GFP target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac Dual PNG172-STII PLU-HIS

   Plasmid

   #112992

   Purpose

   Baculovirus expression of Drosophila png172-Flag and plu-His

   Depositor

   Terry Orr-Weaver

   Inserts

   png172 (png Fly)
   plu (plu Fly)
   

   Use

   Tags

   Flag and His

   Expression

   Insect

   Mutation

   png172 mutation

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac Dual PNG-STII PLU-HIS

   Plasmid

   #112993

   Purpose

   Baculovirus expression of Drosophila png-Flag and plu-His

   Depositor

   Terry Orr-Weaver

   Inserts

   png (png Fly)
   plu (plu Fly)
   

   Use

   Tags

   Flag and His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEM318 - sgRNA mosTI unc-119 single-copy

    Plasmid

    #159822

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 single-copy
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSEM319 - array co-injection fragment mosTI unc-119

    Plasmid

    #159823

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI unc-119
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

    Plasmid

    #125235

    Purpose

    Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

    Plasmid

    #125228

    Purpose

    Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid

    #187956

    Purpose

    FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-hHDAC4-SpdCas9-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid

    #187953

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid

    #187957

    Purpose

    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEM320 - sgRNA mosTI unc-119 array insertion

    Plasmid

    #159824

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 array insertion
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)

    Plasmid

    #107247

    Purpose

    beta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.

    Depositor

    Bryan Roth

    Article

    Roth et al bioRxiv 251769

    Insert

    ARRB2
    

    Use

    AAV

    Tags

    MYC, PYLcs, and YFP

    Expression

    Mutation

    Promoter

    hSYN

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

    Plasmid

    #216247

    Purpose

    Express OsTIR1.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    OsTIR1.F74A
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

    Plasmid

    #194221

    Purpose

    Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge