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Plasmid#82583Purpose5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expressionDepositorInsertEF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-Ef1a-Bamh1
Plasmid#203308PurposeFor expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVH21-Tier1-PhEF1a
Plasmid#169561PurposeTier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-CCNE2
Plasmid#162554PurposeOverexpression of CCNE2DepositorInsertOverexpression of CCNE2 (CCNE2 Human)
UseTagsExpressionMammalianMutationNonePromoterEF1AAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a_flagLaM4-Notch-Gal4VP64
Plasmid#216669PurposeExpresses anti-mCherry synNotch Gal4VP64DepositorInsertanti-mCherry synNotch Gal4VP64
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-iDreV
Plasmid#140136Purposecan be used to generate AAV virus that will express light-inducible site-specific iDreV recombinaseDepositorHas ServiceAAV PHP.eB and AAV1InsertiDreV
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
mc2 mNeon EF1a
Plasmid#206224PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP
Plasmid#155306PurposeRfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFPDepositorInsertRfx Cas13d CRISPR RNA BFP
UseTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1aL-mCherry-W
Plasmid#126703PurposeConstitutive, ubiquitous mCherry expression.DepositorInsertmCherry
UseLentiviralTagsExpressionMutationPromoterEf1aLAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-N-CretrcintG
Plasmid#69570PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - N-CretrcintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112924PurposepiggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-C-CreintG
Plasmid#69571PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1Insertkozak consensus - C-CreintG
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-Usp9x_CD-mCherry
Plasmid#170380PurposeVector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.DepositorInsertUbiquitin specific protease 9x catalytic domain (Usp9x Mouse)
UseTagsmCherry-3xFlagExpressionMammalianMutationCatalytic domainPromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Backbone(NG)
Plasmid#27963DepositorInsertTAL-Backbone(NG)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEF1alpha-mClover3-bGHpA
Plasmid#172286PurposeConstitutive expression of mClover3DepositorInsertmClover3
UseTagsExpressionBacterialMutationPromoterEF1alphaAvailable sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-Ef1a-EcoR1
Plasmid#203311PurposeFor expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9GFP-W
Plasmid#200100PurposeLentiviral vector expressing Cas9 fused with GFP at the C-terminusDepositorInsertGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-LIC
Plasmid#120247PurposeFor LIC cloning with N-terminal V5 tagDepositorTypeEmpty backboneUseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9-iP-A
Plasmid#60599PurposeExpresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertCRISPR Cas9
UseCRISPRTagsExpressionMammalianMutationCodon usage optimized for human usagePromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHeEf1a-mEGFP-NLS
Plasmid#205012PurposeThe vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only