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pcDNA-Cx43-Myc

Plasmid

#60693

Purpose

mammalian expression of Myc-tagged rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43

Plasmid

#60692

Purpose

retroviral expression of rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pF714-pET28a-Hs-FL-METTL20-NHis wt

Plasmid

#85115

Purpose

expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length METTL20 (METTL20 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Bmi1

Plasmid

#61532

Purpose

Expresses Bmi1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Bmi1 (Bmi1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-YA/VD

Plasmid

#60699

Purpose

mammalian expression of rat Cx43 YA/VD mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Tags

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP K45E

Plasmid

#21734

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

Insert

MAP4K3 (MAP4K3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Mutation of the ATP-binding site (K45E). This ver…

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43-YA/VD

Plasmid

#60700

Purpose

retroviral expression of rat Cx43 YA/VD mutant

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

LTR

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pGL4.24-Cd36-enhancer5

Plasmid

#138577

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17893352_17894696

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Minimal promoter

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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His10-B1 Integrin

Plasmid

#186139

Purpose

For expression of recombinant his10-beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Use

Tags

MBP + uncleavable His10

Expression

Bacterial

Mutation

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Arl6ip6

Plasmid

#120235

Purpose

Expresses V5-tagged Arl6ip6 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Arl6ip6 (Arl6ip6 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MAP4K3-GFP d344-873

Plasmid

#21732

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

Insert

MAP4K3 (MAP4K3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus, amino-acids 344…

Promoter

Available since

June 3, 2010

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP d1-343

Plasmid

#21733

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 8. ()

Insert

MAP4K3 (MAP4K3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the amino-terminus, amino-acids 1 to …

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pCDH-CMV-HA-CFP-SLMAP (isoform r)

Plasmid

#235684

Purpose

Express CFP tagged SLMAP gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

SLMAP (SLMAP Human)

Use

Lentiviral

Tags

HA-CFP

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-V5-RFP-SLMAP (isoform r)

Plasmid

#235685

Purpose

Express RFP tagged SLMAP gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

SLMAP (SLMAP Human)

Use

Lentiviral

Tags

V5-RFP

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 JDM37-GFPnb-mCh

Plasmid

#235666

Purpose

Expresses designed activator of Hsp70 tagged with a GFP nano body and mCherry

Depositor

David Baker

Article

Zhang et al Cell Chem Biol. 2025 Feb 4:S2451-9456(25

Insert

JDM37-GFPnb-mCh (PMCH Synthetic, Human)

Use

Tags

GFP nanobody and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF-MjCouRS

Plasmid

#229066

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

Methanococcus jannaschii tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2-sgCBS-1

Plasmid

#230081

Purpose

Crispr knock out human CBS gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

CBS (CBS Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR v2-sgCBS-2

Plasmid

#230082

Purpose

Crispr knock out human CBS gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

CBS (CBS Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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STIM1-2Strep

Plasmid

#210361

Purpose

Expresses STIM1 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

STIM1 (STIM1 Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pSCALPS-FLAG-HA-mmuMettl3 APPA

Plasmid

#207124

Purpose

Lentiviral plasmid to express mouse Mettl3 APPA mutant

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Mettl3 (Mettl3 Mouse)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mutation

D395A and W398A

Promoter

SSFV

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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