We narrowed to 508 results for: transposase

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pMS11: pTns(AvCAST)_ΔTniQ,ΔTnsD

Plasmid

#168144

Purpose

Inducible expression of AvCAST TnsA, TnsB and TnsC proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST Tns proteins (TnsA, TnsB and TnsC)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS9: pTns(AvCAST)_ΔTniQ

Plasmid

#168142

Purpose

Inducible expression of AvCAST TnsA, TnsB, TnsC and TnsD proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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Tn7 helper plasmid

Plasmid

#141161

Purpose

Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

Depositor

Shimon Bershtein

Article

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Insert

Tn7 transposon machinery

Expression

Bacterial

Promoter

Arabinose-inducible pBAD

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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PT2/C-Luc//PGK-SB13

Plasmid

#20207

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

Flirefly luciferase/Sb transposase

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-mSTING

Plasmid

#229739

Purpose

Expresses mouse STING in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

STING (Sting1 Mouse)

Tags

None

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pOPO685

Plasmid

#195300

Purpose

pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

McCAST Cascade and native array with PaqCI entry site

Expression

Bacterial

Promoter

Arabinose inducible arapBAD

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/CMV-Luc/PGK-SB100X

Plasmid

#192869

Purpose

Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

Depositor

Maria Castro, David Largaespada

Article

Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

Inserts

luc
SB100X

Expression

Mammalian

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-HuC:Dendra2

Plasmid

#80904

Purpose

Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

Dendra2 (elavl3 Synthetic)

Use

Zebrafish expression

Promoter

HuC

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

Plasmid

#176531

Purpose

PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

Depositor

Jared Toettcher

Article

Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

Inserts

KGV transcription factor
TagBFP

Use

Piggybac transposase integration vector

Tags

ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

Expression

Mammalian

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-spCas9-SB

Plasmid

#169919

Purpose

Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.

Depositor

Zhili Rong

Article

Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

Insert

SpCas9-SB100X

Use

CRISPR

Promoter

CAG

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET15bHis6Tnp

Plasmid

#79807

Purpose

Transform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutations

Depositor

Keji Zhao

Article

Lai et al Nat Methods. 2018 Sep;15(9):741-747. doi

Insert

His6Tnp

Tags

His

Expression

Bacterial

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSL1749 (pDonor, R*MmeI)

Plasmid

#160733

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA

Expression

Bacterial

Promoter

N/A

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-hTBK1-E696K-EGFP

Plasmid

#221554

Purpose

Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Mutation

Changed glutamate 696 to lysine

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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1154B_gFLE_pB_VTK_ActG

Plasmid

#187238

Purpose

An. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fle

Depositor

Omar Akbari

Article

Smidler et al Sci Adv. 2023 Jul 7;9(27):eade8903. doi:

Insert

Femaleless

Use

CRISPR

Expression

Insect

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHP138 Myc-TRF2-deltaB deltaM

Plasmid

#164255

Purpose

for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TRF2

Tags

Myc

Expression

Mammalian

Mutation

deletion of amino acids-86 and 496-542

Promoter

Tetracycline inducible promotor

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Lifeact-miRFP703

Plasmid

#178530

Purpose

Expresses Lifeact-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Lifeact-miRFP703

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSL0374 (His-MBP-Cas8_Cas7_Cas6)

Plasmid

#130639

Purpose

Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCas8, VchCas7, VchCas6

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on Cas8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTS2356_Tier3(PB)-PuroR

Plasmid

#169655

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSL0373 (His-MBP-TniQ)

Plasmid

#130638

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMS24: pCascade(PmcCAST)_entry

Plasmid

#168157

Purpose

Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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