We narrowed to 6,927 results for: 11

1. pcDNA3-AKAR4-NES

   Plasmid

   #64727

   Purpose

   Cytosol-targeted, FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

   Insert

   AKAR4-NES
   

   Use

   Tags

   Cerulean, NES, and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. AlkB-D135T

   Plasmid

   #160433

   Purpose

   A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

   Depositor

   Tao Pan

   Article

   Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

   Insert

   E. coli AlkB
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 135 to Threonine; deleted t…

   Promoter

   T7

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. ALC1-c005

   Plasmid

   #173092

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   CHD1L (CHD1L Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   L613-K879, codon optimized

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-NGLY1-Puro

   Plasmid

   #181916

   Purpose

   Lentiviral plasmid that directs the expression of NGLY1 in mammalian cells.

   Depositor

   Scott Dixon

   Article

   Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

   Insert

   N-glycanase 1 (NGLY1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ARH1-c003

   Plasmid

   #173093

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   ADPRH (ADPRH Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E2-L357, codon optimized

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MacroH2A.2-c002

   Plasmid

   #173089

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROH2A2 (MACROH2A2 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K167-K372

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. XE118 CAMKII-T286D-CS2+

   Plasmid

   #16736

   Depositor

   Randall Moon

   Article

   Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

   Insert

   CAMKII-T286D (Camk2a Rat)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   threonine 286 mutated to aspartate

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pET28a(+) 6xHis-3xFlag-IFIT5

   Plasmid

   #53561

   Purpose

   bacterial expression of IFIT5

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT5 (IFIT5 Human)
   

   Use

   Tags

   3xFLAG, 6xHIS, and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGEX-4T-1 3xFlag-p38alpha

   Plasmid

   #47575

   Purpose

   Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   p38alpha (Mapk14 Mouse)
   

   Use

   Tags

   3xFlag and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

10. ARH3-c002

    Plasmid

    #173095

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRS (ADPRS Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    A14-S363

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pb459-mU6-sgRNA-EF1a-PuroR

    Plasmid

    #195507

    Purpose

    piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    Puromycin
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. ZNF668-Flag

    Plasmid

    #42636

    Depositor

    Shiaw-Yih Lin

    Article

    Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

    Insert

    ZNF668 (ZNF668 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pGEX-4T-1 3xFlag-ERK2

    Plasmid

    #47573

    Purpose

    Bacterial expression plasmid for GST-3xFlag-tagged ERK2

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    3xFlag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

14. ALC1-c007

    Plasmid

    #173112

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Use

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBluescript GPX-1

    Plasmid

    #13038

    Depositor

    Peter Newburger

    Article

    Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

    Insert

    GPX-1 (GPX1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Additional GCG upstream of codon 11. Codon 92 is …

    Promoter

    Available since

    Nov. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pHis-zfEpoa

    Plasmid

    #65611

    Purpose

    Express soluble His-tagged zebrafish erythropoietin in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

    Insert

    erythropoietin (epoa Zebrafish)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD/HisB-Medium-FT

    Plasmid

    #31917

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    araB

    Available since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. (326) pAAV SV40-ZsGreen U6-gRNA

    Plasmid

    #163020

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. mSecPrP-pCB6+

    Plasmid

    #13917

    Depositor

    Susan Lindquist

    Article

    Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

    Insert

    PrP (Prnp Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    3F4 epitag

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pT7-EGFP-C1-HsXRN1_1174-1706_K

    Plasmid

    #146788

    Purpose

    Mammalian Expression of HsXRN1_1174-1706

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsXRN1_1174-1706 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA4-Myc-eIF3f

    Plasmid

    #35975

    Depositor

    Susana Valente

    Article

    Valente et al Proc Natl Acad Sci U S A. 2009 Mar 17;10

    Insert

    eukaryotic translation initiation factor 3, subunit F (EIF3F Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKIF1C_R169W-GFP

    Plasmid

    #185981

    Purpose

    Mammalian expression of human KIF1C with R169W mutation and GFP tag.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    R169W - mutation causing HSP (SPG58)

    Promoter

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21a - SodA

    Plasmid

    #89612

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase A
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLMB757

    Plasmid

    #83792

    Purpose

    Fructose biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL0489 (frcB) containing all of RL0488 (frcR)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Pb-TRE3G-TYR-HygR

    Plasmid

    #195508

    Purpose

    inducible Piggybac vector expressing human TYR CDS with hygromycin selection

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    TYR (TYR Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLMB713

    Plasmid

    #83785

    Purpose

    Salicylic acid biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    Region upstream RL1329 (salA) containing all of RL1328 (salR)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET21a - APX

    Plasmid

    #89618

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Ascorbate dependent Tryparedoxin peroxidase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. (401) pAAV CMV-ZsGreen U6-gRNA

    Plasmid

    #163021

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Affibody EGFR:1907.BirA*-pET301/NT-DEST

    Plasmid

    #174691

    Purpose

    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*

    Depositor

    Jean Gariepy

    Article

    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

    Insert

    Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
    

    Use

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    R118G mutation in BirA

    Promoter

    T7 promoter

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Foxn1-pBSII SK

    Plasmid

    #41032

    Depositor

    Nancy Manley

    Article

    Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

    Insert

    Foxn1 in situ probe (Foxn1 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    T7 sense; T3 antisense

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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