We narrowed to 11,278 results for: Vars;

1. pDONR223_PRKCZ_WT

   Plasmid

   #82228

   Purpose

   Gateway Donor vector containing PRKCZ , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PRKCZ (PRKCZ Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR207 SARS-CoV-2 NSP3 Cys857Ala

   Plasmid

   #154027

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP3
   

   Use

   Gateway-compatible entry vector, with mutagenized…

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   June 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1 SARS-CoV-2 S D614G-trunc

   Plasmid

   #158077

   Purpose

   Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223 SARS-CoV-2 N_nostop

   Plasmid

   #152987

   Purpose

   Gateway-compatible Entry vector, with insert of N gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   N
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDONR223_PPP6C_p.R264C

   Plasmid

   #81341

   Purpose

   Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PPP6C (PPP6C Human)
   

   Use

   Gateway entry vector

   Mutation

   R264C

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPGK-T7/2-CD44v6 (-cyt)

   Plasmid

   #137817

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6 (-cyt) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region, R296K (please see dep…

   Promoter

   PGK

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS1424

   Plasmid

   #29220

   Purpose

   The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple155-EGFP-NLS
   

   Use

   High copy number homologous recombination plasmid…

   Expression

   Mammalian

   Promoter

   MiniPromoter Ple155 from the human PCP2 gene

   Available Since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Flag-RNF168 delta RING

   Plasmid

   #133982

   Purpose

   mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of RING

   Promoter

   CMV

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pDONR223_MAGEC2_WT

   Plasmid

   #81862

   Purpose

   Gateway Donor vector containing MAGEC2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   MAGEC2 (MAGEC2 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.CAG.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106202

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

    Plasmid

    #114087

    Purpose

    U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for FnCas12a crRNAs
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. plenti-CAG-IKZF2-V2-FLAG-IRES-GFP

    Plasmid

    #69049

    Purpose

    lentiviral expression of IKZF2 variant 2 with FLAG tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF2 (IKZF2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    CAG

    Available Since

    Oct. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-HLA-B0702-His

    Plasmid

    #104849

    Purpose

    HLA-B0702 his tagged

    Depositor

    Sébastien Walchli

    Article

    Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

    Insert

    major histocompatibility complex, class I, B (HLA-B Human)
    

    Use

    Entry vector for gateway system

    Tags

    his

    Promoter

    none

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop

    Plasmid

    #154029

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP3
    

    Use

    Gateway-compatible entry vector, with mutagenized…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    June 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pEMS2242

    Plasmid

    #111897

    Purpose

    ssAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple321 (TUBB3 Human)
    

    Use

    AAV

    Promoter

    Ple321 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Mouse Alpha2 delta-3 HA pMT2

    Plasmid

    #58728

    Purpose

    expression of HA tagged mouse Alpha2 delta-3 in mamalian cells

    Depositor

    Annette Dolphin

    Article

    Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

    Insert

    alpha2 delta-3 (Cacna2d3 Mouse)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139088

    Purpose

    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9 3.7
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    A262T, R324L,S409I, E480K, E543D, M694I, E1219V

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-TRE3G_3xFLAG-PGC1a4_CMV-mCherry:T2A:Hygro

    Plasmid

    #154263

    Purpose

    Dox/Tet-inducible expression of the Human PGC-1a4 splice variant in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    3xFLAG-PGC-1a4 splice variant (PPARGC1A Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-cT3G-cERP2-ELK1

    Plasmid

    #192918

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of ELK1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    ELK1 (ELK1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR223 SARS-CoV2 TEV-E

    Plasmid

    #154418

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of e…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139091

    Purpose

    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dSpCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PLL5.0-Anillin ShRNA-GFP-AnillinFL

    Plasmid

    #187272

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    U6, CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

    Plasmid

    #169469

    Purpose

    Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDONR223_RNF212_WT

    Plasmid

    #81860

    Purpose

    Gateway Donor vector containing RNF212 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RNF212 (RNF212 Human)
    

    Use

    Gateway entry vector

    Mutation

    W4L

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR223 SARS-CoV2 TEV-M

    Plasmid

    #154419

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of m…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pAAV.GFAP.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106195

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    GFAP

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV.CAG.SF-Venus-iGluSnFR.S72A

    Plasmid

    #106203

    Purpose

    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.S72A
    

    Use

    AAV

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

    Promoter

    CAG

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR223_PPP6C_p.E180K

    Plasmid

    #81334

    Purpose

    Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PPP6C (PPP6C Human)
    

    Use

    Gateway entry vector

    Mutation

    E180K

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge