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Addgene

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We narrowed to 8,355 results for: BLI;

Showing: 7961 - 8000 of 8355 results

  2. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid
    #115698
    Purpose
    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae
    Depositor
    Eckhard Boles
    Article
    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10
    Insert
    SSKar2-sfpHluorin-HDEL
    Use
    Tags
    ER retention signal containing HDEL and ER signal…
    Expression
    Yeast
    Mutation
    for introduced mutations see publication
    Promoter
    MET25
    Available since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    115698-plasmid-map-sequence-id-223642

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  7. pPEE17

    Plasmid
    #128361
    Purpose
    Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Insert
    Acetamidase (amdS)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    TEF1
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    128361-plasmid-map-sequence-id-267942

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  9. pML2(−)WF

    Plasmid
    #108337
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108337-plasmid-map-sequence-id-209244

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  10. pML2(+)BR

    Plasmid
    #108336
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108336-plasmid-map-sequence-id-214578

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  11. pML2(+)WF

    Plasmid
    #108333
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108333-plasmid-map-sequence-id-209522

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  12. pML2(+)BF

    Plasmid
    #108334
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108334-plasmid-map-sequence-id-209526

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  13. pML2(+)WR

    Plasmid
    #108335
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108335-plasmid-map-sequence-id-209243

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  14. pML2(−)BF

    Plasmid
    #108338
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108338-plasmid-map-sequence-id-209524

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  15. pML2(−)WR

    Plasmid
    #108339
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108339-plasmid-map-sequence-id-209534

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  16. pML2(−)BR

    Plasmid
    #108340
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108340-plasmid-map-sequence-id-209245

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  22. pJOG1368

    Plasmid
    #167301
    Purpose
    Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Insert
    FCY-UPP fusion gene
    Use
    Synthetic Biology; Moclo level 0 vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    167301-plasmid-map-sequence-id-333328

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  23. pJOG1336

    Plasmid
    #167303
    Purpose
    Level 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Insert
    3'UTR and Terminator SlOLE2
    Use
    Synthetic Biology; Moclo level 0 vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    167303-plasmid-map-sequence-id-333334

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  24. pJOG1334

    Plasmid
    #167302
    Purpose
    Level 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Insert
    Promoter SlOLE2
    Use
    Synthetic Biology; Moclo level 0 vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    167302-plasmid-map-sequence-id-333330

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  28. pJRoC30_VP8H

    Plasmid
    #188062
    Purpose
    PROSS-designed versatile peroxidase 8H
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35
    Insert
    PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
    Use
    Tags
    S. cerevisiae native α-factor prepro-leader signa…
    Expression
    Yeast
    Mutation
    38 PROSS mutations, described in publication
    Promoter
    Available since
    Aug. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    188062-plasmid-map-sequence-id-370147

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  30. pCfB2793

    Plasmid
    #64043
    Purpose
    pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.
    Depositor
    Irina Borodina, Jerome Maury
    Article
    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi
    Insert
    GFP
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 promoter and none
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    64043-plasmid-map-sequence-id-149101

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  34. CAG sHRPb-FKBP-post mGRASP

    Plasmid
    #73146
    Purpose
    Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPb-FKBP-post mGRASP
    Use
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73146-plasmid-map-sequence-id-134519

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  35. pLenti-TetO-PM

    Plasmid
    #197387
    Purpose
    To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)
    Depositor
    Qiao Zhou
    Article
    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038
    Insert
    PDX1-T2A-MAFA
    Use
    Lentiviral; Teto
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    197387-plasmid-map-sequence-id-385579

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Showing: 7961 - 8000 of 8355 results