We narrowed to 4,224 results for: Lta;

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pGEX6p-1-OSF-UFM1

Plasmid

#204565

Purpose

Expresses GST-Strep-FLAG-tagged UFM1 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFM1 (UFM1 Human)

Expression

Bacterial

Mutation

delta84-85aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-SMAC-HA-eGFP

Plasmid

#67492

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pQTEV-ILKAP

Plasmid

#34817

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

integrin-linked kinase-associated serine/threonine phosphatase 2C (ILKAP Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag MFF iso1 WT

Plasmid

#74388

Purpose

Flag tagged human MFF isoform 1

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

delta exon 1 (1-26)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
YFP-synDAT-S/A

Plasmid

#48792

Purpose

Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygro

Depositor

Jonathan Javitch

Article

Fog et al Neuron. 2006 Aug 17;51(4):417-29.

Insert

YFP-synhDAT-S/A (SLC6A3 Human)

Tags

YFP

Expression

Mammalian

Mutation

Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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YFP-synDAT-S/D

Plasmid

#48793

Purpose

Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygro

Depositor

Jonathan Javitch

Article

Fog et al Neuron. 2006 Aug 17;51(4):417-29.

Insert

YFP-synhDAT-S/D (SLC6A3 Human)

Tags

YFP

Expression

Mammalian

Mutation

Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV3-SMAC-HA-eGFP

Plasmid

#67489

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
Kv7.4 (1-645, Δ368-523)/pcDNA3.1

Plasmid

#111456

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523), C643A

Available Since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV2-SMAC-HA-eGFP

Plasmid

#67488

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV5-SMAC-HA-eGFP

Plasmid

#67491

Purpose

Targets HA-eGFP to mitochondrial intermembrane space (IMS)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SMAC (DIABLO Human)

Tags

HA-eGFP

Expression

Mammalian

Mutation

aa1-59

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pSFG-TFII-I-GFP Y248&249F

Plasmid

#22196

Depositor

Ananda Roy

Article

Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Changed Tyrosines 248 and 249 to Phenylalanines. …

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pSP64-hERG1a F656A

Plasmid

#53054

Purpose

Expresses alpha subunit of F656A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Phenylalanine 656 to Alanine

Promoter

SP6

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a Y652A

Plasmid

#53053

Purpose

Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Tyrosine 652 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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MZB131

Plasmid

#217839

Purpose

Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged AP3D1 adaptin appendage (920-1153) for pulldown assays

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

AP3D1 (AP3D1 Human)

Tags

10x His, HaloTag, and SUMO (SMT3)

Expression

Bacterial

Mutation

Deleted residues 1-919

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8

Plasmid

#209048

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF3267

Plasmid

#144743

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SNAPC2 (SNAPC2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 RPS6KC1 MIT, residues 235-316

Plasmid

#180622

Purpose

Bacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

RPS6KC1 (RPS6KC1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 235-316

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2C-ΔC

Plasmid

#200555

Purpose

Mammalian expression of secreted mouse CES2C

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C delta C-terminal (Ces2c Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-ShadowY-CLC

Plasmid

#193552

Purpose

Expresses mouse Clathrin Light Chain A tagged with mEGFP and ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain A (Clta Mouse)

Tags

ShadowY and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R1CiM_4EGFP_R3EGFPi2

Plasmid

#185968

Purpose

Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Molecular IMPLY gate

Use

Synthetic Biology

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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