We narrowed to 5,622 results for: crispr cas9 grna plasmid

1. SlugCas9-HF

   Plasmid

   #163796

   Purpose

   Expresses highly specific SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   SlugCas9 (R247A, N415A, T421A, R656A)

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

   Plasmid

   #60230

   Purpose

   Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   EGFP
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA-P2A and EGFP-KASH

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hSyn

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV.U6.gLacZ.Cas9-T2A-GFP

   Plasmid

   #170361

   Purpose

   Negative control plasmid used for Crispr/cas9 based disruption.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

   Insert

   Cas9-T2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)

   Plasmid

   #209198

   Purpose

   Mutagenesis of Th

   Depositor

   Larry Zweifel

   Article

   Yu et al Neuron. 2021 Apr 21;109(8):1365-1380.e5.

   Insert

   Th (Th Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

   Plasmid

   #209197

   Purpose

   Mutagenesis of Faah

   Depositor

   Larry Zweifel

   Article

   Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

   Insert

   Faah (Faah Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPR-S12

   Plasmid

   #84031

   Purpose

   To episomally express codon optimized Cas9 and chimeric guide RNA

   Depositor

   Sen Wu

   Article

   Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

   Inserts

   hSpCas9
   eGFP
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and CMV (downstream of F2A self-cleaving pept…

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pH-STEME-NG-esgRNA

   Plasmid

   #138136

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice

   Depositor

   Caixia Gao

   Insert

   APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pH-STEME-1-esgRNA

   Plasmid

   #138135

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice

   Depositor

   Caixia Gao

   Insert

   APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

   Plasmid

   #78606

   Purpose

   pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   173CMV, U6

   Available since

   Dec. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

10. gRNA-hIRF-1 #12/pSIR-hCD2

    Plasmid

    #135392

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    gRNA_hIRF1 promoter #12
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330-gRNA

    Plasmid

    #158973

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

    Depositor

    Charles P. Lai

    Article

    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

    Insert

    gRNA3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SunTag-FWAgRNA-4-14aa-TET1cd

    Plasmid

    #106436

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS (TET1 Mustard Weed, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LLP109 pEF1a-gRNA-GFP

    Plasmid

    #100935

    Purpose

    GFP, puromycin and gRNA scaffold driven by EF1a promoter

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA scaffold
    

    Use

    Grna scaffold

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

    Plasmid

    #223163

    Purpose

    2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Nsp2Cas9_AAV

    Plasmid

    #192143

    Purpose

    Expresses Nsp2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

    Insert

    Nsp2Cas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NarCas9_AAV

    Plasmid

    #192145

    Purpose

    Expresses NarCas9?and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

    Insert

    NarCas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid

    #170182

    Purpose

    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    mCherry
    

    Use

    Tags

    3XFlag and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. B-HiFi SpCas9

    Plasmid

    #207362

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiCRISPR v2

    Plasmid

    #52961

    Purpose

    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pACRISPR

    Plasmid

    #113348

    Purpose

    A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge