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pNA 3C myc scfvHA

Plasmid

#234991

Purpose

Expression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteins

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

NA-3C-myc-scfvHA

Expression

Mammalian

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-BLRR

Plasmid

#158958

Purpose

Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

BLRR

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMax_ScaI_BFP

Plasmid

#177824

Purpose

Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

tagBFP

Expression

Mammalian

Mutation

5' inserted ScaI RE cut site

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA

Plasmid

#176540

Purpose

Mammalian expression of nuclear localized OgeuIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

OgeuIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHPHS232

Plasmid

#228356

Purpose

Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Inserts

mTagBFP2
Reverse tetracycline transactivator 3
neo

Tags

Blasticidin S deaminase and iCasp9

Expression

Mammalian

Promoter

TRE3GV and gene trap

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDule-Tet2.0

Plasmid

#85496

Purpose

Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

Insert

Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Tags

None

Expression

Bacterial

Mutation

Y32G L65Q F108S Q109D D158S L162N

Promoter

lpp (constitutive)

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-mPGK-eGFP (beta-globin intron)

Plasmid

#162513

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

mPGK

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-SpdCas9-W3SL

Plasmid

#210708

Purpose

This Plasmid express hSyn promoter driven SpdCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

S. Pyogenes dCas9

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

D10A/H840A

Promoter

hSyn

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-IgK-pHluorin-TM-mRuby

Plasmid

#185546

Purpose

Expresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

IgK-pHluorin-TM-mRuby

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF1305

Plasmid

#143785

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZMIZ1 (ZMIZ1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-CCR1

Plasmid

#222311

Purpose

Synchronize the trafficking of CCR1 from the ER.

Depositor

Franck Perez

Article

Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

Insert

Streptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GAL4-iLight-VP16

Plasmid

#170274

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16

Use

AAV

Mutation

See Comments

Promoter

CaMKII

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RA-HA

Plasmid

#46859

Purpose

expresses mouse IL-17RA

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA (Il17ra Mouse)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pMis1_1B

Plasmid

#192079

Purpose

A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

Depositor

Markus Buchhaupt

Article

Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

Inserts

KanR
rhaR
rhaS
pBBR1 rep

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 P.1

Plasmid

#173476

Purpose

Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 Spike Truncated to remove 18 amino acids

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGG-9.47_VP1,3 #2515

Plasmid

#74237

Purpose

Modified plasmid with a different backbone that yields higher of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP1
AAV9.47 VP3

Use

AAV

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RC(FL)

Plasmid

#46864

Purpose

expresses mouse IL-17RC (all exons)

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RC (Il17rc Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pGG-9.47_VP2_AS #2562

Plasmid

#74238

Purpose

Modified plasmid with a different backbone that yields higher titers of AAV-AS vectors. Encodes VP2-AS chimeric protein with 19 alanines fused to N-terminus of AAV9.47 VP2.

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV9.47 VP2-AS

Use

AAV

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSF4 CMV 5TOP intron renilla TRICK CTE polyA

Plasmid

#64544

Purpose

5′ TOP TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Tags

24xMS2 stem loops, 5' TOP (5′ terminal oligo…

Expression

Mammalian

Promoter

Tetracycline-inducible promoter and 5′ UTR of hum…

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV CytoBAS

Plasmid

#62860

Purpose

Fluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

CytoBAS

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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