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Plasmid#202340PurposeDesign 70.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert70.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
70.b_pSTC0-zeo
Plasmid#202341PurposeDesign 70.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert70.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
50.a_pSTC0-zeo
Plasmid#202342PurposeDesign 50.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert50.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
50.b_pSTC0-zeo
Plasmid#202343PurposeDesign 50.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert50.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
rw-consensus_pSTC0-zeo
Plasmid#202349PurposeControl rw-consensus (reweighted consensus) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsertrw-consensus
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
98.a_pSTC0-zeo
Plasmid#202332PurposeDesign 98.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert98.a
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
98.b_pSTC0-zeo
Plasmid#202333PurposeDesign 98.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassetteDepositorInsert98.b
UseTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121-Split-TALED
Plasmid#218171PurposeExpress plastid TALE-linked deaminases (TALED), composed of TALE, split DddA originated from Burkholderia cenocepacia, and an engineered deoxyadenosine deaminase derived from the E. coli TadA proteinDepositorInsertTALE-Intron-N-DddAtox half (G1397N)-T2A-TALE-C-DddAtox half (G1397C)-TadA8e
UsePlastid base editingTagsExpressionPlantMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD118A
Plasmid#218140PurposeExpresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coliDepositorInsertSD0_ZF2-3
UseTagsAviTag-6xHis-MBP-TEV-(GGGGS)2ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD068F
Plasmid#218137PurposeLentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.DepositorInsertSD36-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD057C
Plasmid#218136PurposeFor hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved ZF degron SD36.DepositorInsertSD36
UseTagsrpoZExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD113A
Plasmid#218133PurposeFor hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56DepositorInsertSD56
UseTagsrpoZExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM154
Plasmid#218121PurposeHybrid circuit displaying FKBP12 bait and driving expression of luxABDepositorInsertsFKBP12
luxAB
UseLuciferaseTags434cI_RR69ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-GST-TEV-NAP1
Plasmid#217610PurposeFor expression in E. coli of NAP1DepositorInsertNAP1 (AZI2 Human)
UseTagsGSTExpressionBacterialMutationPromoterTAC promoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-GST-TEV-SINTBAD-mCherry
Plasmid#208874PurposeFor expression in insect cells of SINTBAD-mCherryDepositorInsertSINTBAD (TBKBP1 Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTcI38-PRM-wasabi
Plasmid#195554PurposeTemperature-dependent expression of mWasabi reporter with transcription activation by TcI38DepositorInsertsmWasabi
TcI38
UseTags6x His and AANDENYADAS degradation tagExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188GPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCLV3Cas-LT2
Plasmid#210550PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporterDepositorInsertCLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT2::mCherrySSM
UseTagsExpressionPlantMutation1bp insertion after AAVS1 target sequence that di…PromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211844PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter
Plasmid#211847PurposePlant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degronDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoter35SAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:TDimer mVenus
Plasmid#210520PurposeExpresses a tandem dimer of mVenus in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertTandem dimer mVenus
UseZebrafish expression, tol2 kit gateway-compatibleTagsExpressionMutationPromoterZebrafish insulin promoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only