Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 10,444 results for: T7

Showing: 8001 - 8040 of 10444 results

  2. pET-scFv-T

    Plasmid
    #67843
    Purpose
    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.
    Depositor
    Joanna Bereta
    Article
    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24
    Insert
    antibody 1D8 light kappa chain
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67843-plasmid-map-sequence-id-117725

    Enlarge

  4. pcDNA3.1.Gα13-LgB106

    Plasmid
    #134364
    Purpose
    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gα13-LgB106 (GNA13 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134364-plasmid-map-sequence-id-306388

    Enlarge

  13. pcDNA3.1.Gαi1-LgB91

    Plasmid
    #134342
    Purpose
    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi1-LgB91 (GNAI1 Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134342-plasmid-map-sequence-id-306400

    Enlarge

  14. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184225-plasmid-map-sequence-id-361604

    Enlarge

  20. pLKO-TRE-AFOS

    Plasmid
    #197079
    Purpose
    A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.
    Depositor
    Elaine Fuchs
    Article
    Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1
    Insert
    AFOS (Fos Mouse)
    Use
    Lentiviral
    Tags
    Flag and T7
    Expression
    Mutation
    Promoter
    TRE
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    197079-plasmid-map-sequence-id-396633

    Enlarge

  21. pBtPIPLC His

    Plasmid
    #173808
    Purpose
    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Anne Gershenson, Mary F Roberts
    Article
    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4
    Insert
    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    Use
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    173808-plasmid-map-sequence-id-345681

    Enlarge

  38. SSTR3-DuET

    Plasmid
    #213378
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR3 (SSTR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213378-plasmid-map-sequence-id-430734

    Enlarge

  39. pET23a-H6-TEV-cSrc

    Plasmid
    #214233
    Purpose
    Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings
    Depositor
    Neel Shah
    Article
    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1
    Insert
    cSrc Kinase domain (SRC Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214233-plasmid-map-sequence-id-422793

    Enlarge

Showing: 8001 - 8040 of 10444 results