We narrowed to 24,461 results for: nes

1. Venus-Cdc20 R132A (1768)

   Plasmid

   #26059

   Depositor

   Jonathon Pines

   Article

   Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

   Insert

   Cdc20 (CDC20 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Mutation in the Mad2 binding site, R132A

   Promoter

   Available since

   Aug. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

2. p6xAP-1_RE

   Plasmid

   #118054

   Purpose

   GBPart - Operator/DNA Response element - 6 copies of the AP-1 binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   6 copies of the AP-1 binding motif
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. YFP(1-158) in pcDNAI/Amp

   Plasmid

   #60888

   Purpose

   This vector attaches YFP(1-158) to the N-terminus of a protein.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(1-158)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met was substituted for Gln-69.

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUPD_OsU3 (GB1184)

   Plasmid

   #68209

   Purpose

   Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   OsU3 promoter
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 shPIK3CD.v1 puro

   Plasmid

   #58706

   Purpose

   Lentiviral shRNA vector for knockdown of human PIK3CD

   Depositor

   Kevin Janes

   Article

   Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

   Insert

   shPIK3CD (PIK3CD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

6. TB:2xP53_RE:MiniP

   Plasmid

   #133881

   Purpose

   2 copies of the p53 DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   2 copies of the p53 DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPAtUbq10 (GB0223)

   Plasmid

   #68174

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq10 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SEPLuc-IRES-Antares

   Plasmid

   #141085

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   SEPLuc-EMCV IRES-Antares
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pG418R-GMRWhite

   Plasmid

   #165883

   Purpose

   G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Transgenesis Ready Selection Vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPAct2noUTR (GB0192)

    Plasmid

    #68177

    Purpose

    Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtAct2 promoter (no UTR)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YFP(159-238) in pcDNAI/Amp

    Plasmid

    #60889

    Purpose

    This vector attaches YFP(159-238) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

    Insert

    YFP(159-238)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSTAP-seq_fly-zfh1

    Plasmid

    #86381

    Purpose

    Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer
    

    Use

    Stap-seq screening vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHcPro (GB0067)

    Plasmid

    #68196

    Purpose

    Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    TEV HcPro coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Antares-SEPLuc

    Plasmid

    #141086

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 66 to 91 on the Luciferas…

    Promoter

    CMV

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. K347CLM G234A/43/347

    Plasmid

    #24454

    Depositor

    Ron Vale

    Article

    Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, G234A/S43C/E3…

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pVenus-N1 cyclin B1 L45A (1324)

    Plasmid

    #61851

    Purpose

    mammalian expression of cyclin B1 L45 A mutant fused to Venus

    Depositor

    Jonathon Pines

    Article

    Matsusaka et al Biol Open. 2014 Sep 12;3(10):904-12. doi

    Insert

    cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    L45A

    Promoter

    CMV

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDGE76

    Plasmid

    #80578

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCerulean3-H2B-C-10

    Plasmid

    #55422

    Purpose

    Localization: Nucleus/Histones, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pUC 3GLA UAS HAi

    Plasmid

    #71763

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOT2 3GLA

    Plasmid

    #71764

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge