We narrowed to 7,456 results for: PAC

1. pcDNA5-FRT-TO-eGFP

   Plasmid

   #157733

   Purpose

   mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T-CMV-evoCjCas9-BE4max-BlastR

   Plasmid

   #194061

   Purpose

   Mammalian expression of evoCjCas9 CBE4max

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-BE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-dSa-VPR mini.-2X snRP1

   Plasmid

   #99688

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. dSp-VP64-p65(100-261)-RTA(125-190)

   Plasmid

   #99673

   Purpose

   Expresses dSp Cas9 fused to combination of truncated activation domains

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   Tags

   VP64-p65(101-261)-RTA(125-190)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRIP-CMV-mTagBFP2-2A

   Plasmid

   #102602

   Purpose

   Control lentivector. Coexpression of mTagBFP and gene of interest from CMV promoter

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTE5400_pMBP_CDS dropout vector

   Plasmid

   #221189

   Purpose

   Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

   Depositor

   Tobias Erb

   Article

   Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

   Type

   Empty backbone

   Use

   Tags

   10x His-tag + E. coli maltose binding protein (MB…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ133-RZ-Cas12j2

   Plasmid

   #189784

   Purpose

   Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM552

   Plasmid

   #154131

   Purpose

   Expresses wt rRNAs operon in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   wt bacterial rRNAs operon under control of pL promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVITRO-HPV31 L1L2

   Plasmid

   #52600

   Purpose

   Expresses HPV31 L1 and L2 in mammalian cells

   Depositor

   Richard Roden

   Article

   Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

   Inserts

   HPV31 L1
   HPV31 L2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSB1K3_M13ps_LacZα_gIII

    Plasmid

    #190435

    Purpose

    M13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.

    Depositor

    Manish Kushwaha

    Article

    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

    Inserts

    M13 packaging signal
    M13 gIII
    LacZα
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-mF(3230)A3

    Plasmid

    #168016

    Purpose

    Expresses MBP-F(3230)A3-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    MBP-F(3230)A3-6xHis
    

    Use

    Tags

    Hisx6 and maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVITRO-HPV35 L1L2

    Plasmid

    #52492

    Purpose

    Expresses HPV35 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV35 L1
    HPV35 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-P3-evoCjCas9-eAID_gRNA

    Plasmid

    #202562

    Purpose

    P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-eAID
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f

    Plasmid

    #72304

    Purpose

    Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

    Depositor

    Adam Cohen

    Article

    Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

    Insert

    CaViar
    

    Use

    Lentiviral

    Tags

    TS

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDAI-SceI-SacB

    Plasmid

    #113635

    Purpose

    Broad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacB

    Depositor

    Miguel Valvano

    Article

    Aubert et al Methods Mol Biol. 2014;1197:311-27. doi:

    Insert

    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Please see Depositor Comments

    Promoter

    sacB promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL-SaBE3

    Plasmid

    #85169

    Purpose

    Expresses SaBE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    SaBE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-E(4130)A2

    Plasmid

    #168021

    Purpose

    Expresses E(4130)A2-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    E(4130)A2-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPBCAG-rtTAM2-IH

    Plasmid

    #140827

    Purpose

    Expresses rtTAM2-IRES-HygR in mammalian cells

    Depositor

    Hideyuki Okano

    Article

    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

    Inserts

    rtTAM2
    HygR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. HBT_pcoCASphi_version1

    Plasmid

    #197931

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    FLAG tag and SV40 NLS and nucleoplasmin NLS

    Expression

    Plant

    Mutation

    Promoter

    35SPPDK promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVITRO-HPV53 L1L2

    Plasmid

    #52599

    Purpose

    Expresses HPV53 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV53 L1
    HPV53 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge