We narrowed to 9,193 results for: Pol;

1. pGM9

   Plasmid

   #220167

   Purpose

   Expresses a reporter for an alpha-helical OMM protein

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   FUNDC1 (FUNDC1 Human)
   

   Use

   Lentiviral

   Tags

   GFP11

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ubc-c005

   Plasmid

   #216876

   Purpose

   Bacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion

   Depositor

   Lari Lehtiö

   Article

   Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

   Insert

   Ubiquitin (UBC Human)
   

   Expression

   Bacterial

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ubc-c004

   Plasmid

   #216875

   Purpose

   Bacterial expression vector for E. coli producing ubiquitin with N-terminal CFP fusion

   Depositor

   Lari Lehtiö

   Article

   Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

   Insert

   Ubiquitin (UBC Human)
   

   Expression

   Bacterial

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGM295

   Plasmid

   #220183

   Purpose

   Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   SEC61B (SEC61B Human)
   

   Use

   Lentiviral

   Tags

   sfGFP

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2701-AGER-tdTomato

   Plasmid

   #216470

   Purpose

   donor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   tdTomato
   

   Use

   CRISPR

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP

   Plasmid

   #216476

   Purpose

   lentiviral dual expression of dsRed and tagBFP

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   dsRed
   tagBFP
   

   Use

   Lentiviral

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MCAM-BirA*-HA

   Plasmid

   #214472

   Purpose

   Lentiviral vector for the expression of MCAM-BirA*-HA fusion protein in mammalian cells.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   MCAM (MCAM Human)
   

   Use

   Lentiviral; Gateway

   Tags

   BirA(R118G) and HA tag

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIp128-CUP1-His6-CUbo(K48R)-GFP

   Plasmid

   #212793

   Purpose

   Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   His6-CUbo(K48R)-GFP
   

   Use

   Integrative vector

   Expression

   Yeast

   Mutation

   CUb(K48R)

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIp128-CUP1-His6-CUbo(K63R)-GFP

   Plasmid

   #212794

   Purpose

   Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   His6-CUbo(K63R)-GFP
   

   Use

   Integrative vector

   Expression

   Yeast

   Mutation

   CUb(K63R)

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. YIp211-ADH-NUbo-E3(48) aa24-150-VSV

    Plasmid

    #212747

    Purpose

    Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(48) aa24-150
    

    Use

    Integrative vector

    Tags

    NUbo and VSV

    Expression

    Yeast

    Mutation

    Cue1 ΔU7BD

    Promoter

    ADH1

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

    Plasmid

    #212738

    Purpose

    Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    E3(1)
    

    Use

    Integrative vector

    Tags

    CUbo-NLS-VSV and myc

    Expression

    Yeast

    Mutation

    HOIP(C885A)

    Promoter

    ADH1

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

    Plasmid

    #212805

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
    

    Expression

    Mammalian

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PABPC1_544-626_pGEX6P1

    Plasmid

    #209790

    Purpose

    Bacterial expression of PABPC1 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al J Biol Chem. 2010 Apr 30;285(18):13599-6

    Insert

    Mlle domain of PABPC1 (PABPC1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 544-626 only

    Promoter

    tac

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. rUBR5_2383-2442_pGEX6P1

    Plasmid

    #209791

    Purpose

    Bacterial expression of UBR5 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

    Insert

    Mlle domain of UBR5 (Ubr5 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2383-2442 only

    Promoter

    tac

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. LARP1_323-509

    Plasmid

    #209800

    Purpose

    Bacterial expression of human LARP1 La-module

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 La-module (LARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-509 only

    Promoter

    T7

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. LARP1_323-410

    Plasmid

    #209801

    Purpose

    Bacterial expression of human LARP1 LaM domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

    Insert

    LARP1 LaM (LARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 323-410 only

    Promoter

    T7

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SUMO-Twinkle 43-372

    Plasmid

    #205054

    Purpose

    TWNK protein expression

    Depositor

    Smita Patel

    Article

    Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

    Insert

    TWNK (TWNK Human)
    

    Expression

    Bacterial

    Mutation

    Lacks Mitochondrial localization signal and C ter…

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV6-AN-HA_aa 321-843 REV1

    Plasmid

    #201587

    Purpose

    Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA repair protein REV1 (REV1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Expresses amino acids 321-843 of DNA repair prote…

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)

    Plasmid

    #201545

    Purpose

    Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks N-terminal residues 1-79

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

    Plasmid

    #201546

    Purpose

    Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 188-228

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

    Plasmid

    #201548

    Purpose

    Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks C-terminal residues 364-409

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBactin-AcGFP-CI

    Plasmid

    #196849

    Purpose

    Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET19-TdTdelta5cys

    Plasmid

    #207465

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    TdTdelta5cys (Dntt Mouse)
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCMV-3UTR

    Plasmid

    #202550

    Purpose

    Ready for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation media

    Depositor

    Rostislav Skrabana

    Article

    Meskova et al Antibodies (Basel). 2023 Aug 4;12(3):51.

    Type

    Empty backbone

    Tags

    ATG initiation codon followed by an IGKV3 signal …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

    Plasmid

    #196877

    Purpose

    Neuron-specific expression of mOrange2 reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBactin-td-mCherry-CI

    Plasmid

    #196851

    Purpose

    Expression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBetaActin-KIF1aMotor-mScarlet-FKBP

    Plasmid

    #191336

    Purpose

    Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    KIF1aMotor-mScarlet-FKBP (Kif1a Mouse)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 1-405

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEE005

    Plasmid

    #176762

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    101
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBetaActin-FKBP-mScarlet-KIFC1motorN593K

    Plasmid

    #191335

    Purpose

    Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-KIFC1N593K (KIFC1 Human)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 125-673; N593K

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. pEE070

    Plasmid

    #176827

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    709
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge