We narrowed to 27,277 results for: Tat

1. pcDNA3-p53-G609C

   Plasmid

   #229533

   Purpose

   Expresses p53 mutant G609C in mamallian cells

   Depositor

   Robin Fåhraeus

   Article

   Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

   Insert

   p53-CDS (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.G609C

   Promoter

   CMV

   Available since

   July 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR_CG6867_nostop

   Plasmid

   #240238

   Purpose

   Gateway entry clone with CG6867 without stop codon

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   CG6867 (CG6867 Fly)
   

   Use

   Gateway shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR_BIP-sfGFP-TurboID-KDEL

   Plasmid

   #240223

   Purpose

   Gateway entry clone with ER-localized TurboID tagged with superfolder GFP (contains stop codon)

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   BIP-sfGFP-TurboID-KDEL
   

   Use

   Gateway shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. H1x

   Plasmid

   #218111

   Purpose

   Encodes mus musculus histone 1 in Gateway entry vector

   Depositor

   Peter Hamilton

   Article

   Kim et al Transl Psychiatry. 2024 Jun 5;14(1):239.

   Insert

   Histone H1x (H1f10 Mouse)
   

   Use

   Gateway cloning entry vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEMHE-Trim21-OLLAS

   Plasmid

   #237438

   Purpose

   Expresses mouse Trim21 ubiquitin ligase tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

   Insert

   Trim21 (Trim21 Mouse)
   

   Use

   Tags

   OLLAS

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-flag-IARS2-Δ1-48

   Plasmid

   #232341

   Purpose

   Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340

   Depositor

   Quanfu Ma

   Insert

   IARS2 (IARS2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-48 (indicated as Mito-signal in UniPro…

   Promoter

   CMV

   Available since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)

   Plasmid

   #238167

   Purpose

   Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   Non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-EGFP/VSVG-rbPodxl

   Plasmid

   #233260

   Purpose

   Expression of EGFP-tagged PODXL WT

   Depositor

   David Bryant, Keith Mostov

   Article

   Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

   Insert

   PODXL (PODXL Rabbit)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. A1-LCD -3D+3N

   Plasmid

   #234614

   Purpose

   Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to asparagine

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1_LCD_3DN (HNRNPA1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   D214N, D242N, D250N

   Promoter

   T7

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. A1-LCD -4D+4N

    Plasmid

    #234611

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to asparagine

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_4DN (HNRNPA1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    D214N, D242N, D250N, D262N

    Promoter

    T7

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. A1-LCD -4D+4V

    Plasmid

    #234610

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to valine

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_4DV (HNRNPA1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    D214V, D242V, D250V, D262V

    Promoter

    T7

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. A1-LCD -3D+3V

    Plasmid

    #234613

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to valine

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_3DV (HNRNPA1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    D214V, D242V, D250V

    Promoter

    T7

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6-CFAPoptim-KAN

    Plasmid

    #221128

    Purpose

    Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS415-TEF1pr-SEC63-FAPoptim

    Plasmid

    #221116

    Purpose

    FAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    FAPoptim-SEC63
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS415-TEF1pr-SEC61-FAPoptim

    Plasmid

    #221115

    Purpose

    FAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC61-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS413-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221095

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GB4635

    Plasmid

    #215263

    Purpose

    Module for the expression of HISPS under the copper inducible CBS:mDFR promoter and H3H under the red light regulated Etr8:CMVmin promoter and the constitutive expression of LUZ, CPH,EGFP and p19

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    CBS4x:mDFR:HISPS-Etr4:mDFR:H3HSF-LUZ-CPH-EGFP-p19
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-hRPL3-FLAG_H245A

    Plasmid

    #233617

    Purpose

    Expression of Flag-tagged hRPL3 with H245A mutation

    Depositor

    Shintaro Iwasaki

    Article

    Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

    Insert

    Flag-tagged hRPL3 with H245A mutation (RPL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H245A

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS413-TEF1pr-ANP1-FAPoptim

    Plasmid

    #221120

    Purpose

    FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    ANP1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS413-TEF1pr-ERG6-FAPoptim

    Plasmid

    #221121

    Purpose

    FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    ERG6-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRS413-TEF1pr-PMA1-FAPoptim

    Plasmid

    #221122

    Purpose

    FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    PMA1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS413-TEF1pr-SEC7-FAPoptim

    Plasmid

    #221124

    Purpose

    FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC7-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS413-TEF1pr-VPH1-FAPoptim

    Plasmid

    #221125

    Purpose

    FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    VPH1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRS415-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221090

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRISPEY-Z3-Nat-ADE2

    Plasmid

    #232106

    Purpose

    Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    Z3

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRISPEY-GAL-Hyg-ADE2

    Plasmid

    #232104

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCRISPEY-Z3-Hyg-ADE2

    Plasmid

    #232107

    Purpose

    Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    Z3

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPB9

    Plasmid

    #210036

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    puro-T2A-eGFP
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-Flag-Jarid2-polyA

    Plasmid

    #223811

    Purpose

    IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

    Depositor

    Azusa Inoue

    Article

    Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

    Insert

    Jarid2 (Jarid2 Mouse)
    

    Use

    In vitro transcription

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDL562

    Plasmid

    #231162

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting SlCHL1

    Depositor

    Daniel Voytas

    Article

    Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

    Insert

    mobile gRNA targeting SlCHL1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDY2259 hNOLC1 atgRNA Paired Guide 1

    Plasmid

    #220989

    Purpose

    hNOLC1 atgRNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

    Insert

    NOLC1 atgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDY2260 hNOLC1 atgRNA Paired Guide 2

    Plasmid

    #220990

    Purpose

    hNOLC1 atgRNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

    Insert

    NOLC1 atgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDY2261 hACTB atgRNA Paired Guide 1

    Plasmid

    #220991

    Purpose

    hACTB atgRNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

    Insert

    ACTB atgRNA (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. act-3p(long):rfp

    Plasmid

    #202330

    Purpose

    The act-3 promoter drives expression of TurboRFP in the pharynx, spermatheca, and body wall of C. elegans.

    Depositor

    Didier Stainier

    Article

    Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

    Inserts

    act-3p(long) promoter
    TurboRFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    This is a promoter sequence for C. elegans act-3 …

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. RhoA gRNA #1

    Plasmid

    #198718

    Purpose

    Cas9-mediated knockout of RhoA in mammalian cells #1

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoA (Rhoa Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RhoC gRNA #2

    Plasmid

    #198725

    Purpose

    Cas9-mediated knockout of RhoC in mammalian cells #2

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    RhoC (Rhoc Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGL3-hL1-gRNA1-EGFP

    Plasmid

    #226002

    Purpose

    CRISPRi-KD of human LINE1

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    sgRNA targeting human L1HS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGL3-hL1-gRNA2-EGFP

    Plasmid

    #226003

    Purpose

    CRISPRi-KD of human LINE1

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    sgRNA targeting human L1HS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. psiCHECK-1-miR-376a1(5p)-WT

    Plasmid

    #215875

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-376a1(5p)-WT (MIR376A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. psiCHECK-1-miR-376a1(3p)-WT

    Plasmid

    #215876

    Purpose

    This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

    Depositor

    Kumiko Ui-Tei

    Article

    Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

    Insert

    three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-WT (MIR376A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge