We narrowed to 17,391 results for: puro

1. p6610 MSCV-IP N-HAonly TIMP1

   Plasmid

   #35009

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   TIMP1 (TIMP1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pAC025

   Plasmid

   #227597

   Purpose

   Lentiviral backbone used when cloning pooled prime editing guide RNA libraries already containing a tevopreQ1 RNA motif

   Depositor

   Brittany Adamson

   Article

   Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXs-Oct3/4-IP

   Plasmid

   #15918

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pD2529-CAG-Flag-mouse TGFb1

   Plasmid

   #180260

   Purpose

   Mouse full length TGFβ1 expression

   Depositor

   Timothy Springer

   Article

   Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

   Insert

   TGFβ1 (Tgfb1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiCRISPRv2-ACTB-C1

   Plasmid

   #169796

   Purpose

   Expresses Cas9 and guide RNA for ACTB gene in human

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   guide RNA for ACTB gene
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMN-009

   Plasmid

   #195413

   Purpose

   AAVS1 Integratable mCherry Reporter for synZiFTR ZF1

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mNeonGreen-Smad3

   Plasmid

   #172098

   Purpose

   Expresses fluorescently tagged human Smad3 in mammalian cells.

   Depositor

   Lea Goentoro

   Article

   Frick et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   Smad 3 (Smad3 Mouse)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   C49S mutation in mCerulean

   Promoter

   CMV

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAC026

   Plasmid

   #227598

   Purpose

   Lentiviral backbone used when cloning pooled prime editing guide RNA libraries that do not already contain a tevopreQ1 RNA motif (backbone contains motif)

   Depositor

   Brittany Adamson

   Article

   Cirincione et al Nat Methods. 2024 Nov 19. doi: 10.1038/s

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPB-cT3G-cERP2-TERF1

   Plasmid

   #222542

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of TERF1

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   TERF1 (TERF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMA3288

    Plasmid

    #46885

    Purpose

    Lentiviral vector encoding Tet-inducible mitochondrially targeted Wild Type human Uracil-N-glycosylase

    Depositor

    Mikhail Alexeyev

    Article

    Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

    Insert

    Uracil-N-glycosylase WT (UNG Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-Tight

    Available since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p663-UBC-miniTurbo-V5-MAPK15_IDG-K

    Plasmid

    #170581

    Purpose

    Gateway destination clone of MAPK15 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MAPK15 (MAPK15 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AsdCas12a KRAB

    Plasmid

    #188502

    Purpose

    Expresses FLAG tagged AsdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PB_TRE_Z18-PDGFR_EF1a_eGFP

    Plasmid

    #186304

    Purpose

    Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z18-PDGFR
    eGFP
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-RT002-LV

    Plasmid

    #163382

    Purpose

    2nd generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEGFRtm (LV)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p667-UBC-BCKDK-V5-miniTurbo_IDG-K

    Plasmid

    #158189

    Purpose

    Gateway destination clone of BCKDK (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    BCKDK-V5-miniTurbo (BCKDK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p663-UBC-miniTurbo-V5-CDK11A_IDG-K

    Plasmid

    #214461

    Purpose

    Gateway destination clone of CDK11A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK11A (CDK11A Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSIQmate

    Plasmid

    #216240

    Purpose

    All-in One IQ-Cumate transgene switch, empty vector

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p667-UBC-ADCK5-V5-miniTurbo_IDG-K

    Plasmid

    #189909

    Purpose

    Gateway destination clone of ADCK5 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    ADCK5 (ADCK5 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Fuw CBX8

    Plasmid

    #121111

    Purpose

    conditional expression

    Depositor

    Emily Dykhuizen

    Article

    Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRIPZ-NLS-PTEN

    Plasmid

    #206984

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLJM1 RagA

    Plasmid

    #73031

    Purpose

    Expresses human RagA from a CMV promoter for stable expression in mammalian cells

    Depositor

    David Sabatini

    Article

    Efeyan et al Dev Cell. 2014 May 12;29(3):321-9. doi:

    Insert

    rragA (RRAGA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLX-UBC_NKX2-1_Short_V5-GFP

    Plasmid

    #221499

    Purpose

    NKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoter

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5-linker-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLKO.1P shSHMT2

    Plasmid

    #106319

    Purpose

    Express shRNA targeting SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    shRNA targeting SHMT2
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

    Plasmid

    #159455

    Purpose

    Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

    Depositor

    Oliver Griesbeck

    Article

    Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

    Insert

    mCRISPRed
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LV-TRE-WT mouse MyoD-T2A-dsRedExpress2

    Plasmid

    #60624

    Purpose

    Expresses WT mouse MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    WT mouse MyoD (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEM705 Gst (Ser)

    Plasmid

    #109354

    Purpose

    GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gst chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMRX-IP-3xFLAG-NCOA4 (dIDR2)

    Plasmid

    #192811

    Purpose

    Stable expression of 3xFLAH-NCOA4 (delta IDR2) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of the IDR2

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MCP-ADAR2-DD

    Plasmid

    #170124

    Purpose

    Lentiviral vector carrying the MCP-ADAR2 with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MCP-ADAR2-DD-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a core promoter

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-Myc-Oct3/4-IP

    Plasmid

    #13460

    Depositor

    Shinya Yamanaka

    Article

    Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Use

    Tags

    Myc (6x)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p663-UBC-V5-CDK13_IDG-K

    Plasmid

    #135268

    Purpose

    Gateway destination clone of CDK13 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-CDK13 (CDK13 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pD2529-CAG-HA-mouse LRRC33

    Plasmid

    #180259

    Purpose

    Mouse full length LRRC33 expression

    Depositor

    Timothy Springer

    Article

    Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

    Insert

    LRRC33 (Nrros Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. E12-pCLBabe

    Plasmid

    #20918

    Depositor

    Stephen Tapscott

    Article

    Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

    Insert

    E12 (TCF3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G155D

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. p6592 MSCV-IP N-HAonly WWP2

    Plasmid

    #34905

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    WWP2 (WWP2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pQC.Flip-nluc(LgBiT1-8)CP[TEV]

    Plasmid

    #200122

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SETMAR-Cas9

    Plasmid

    #186701

    Purpose

    Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETMAR-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Ppst93-ANME2bHR1 MCR-NG

    Plasmid

    #192774

    Purpose

    Expresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. Ppst93-ANME1G37 MCR-NG

    Plasmid

    #192773

    Purpose

    Expresses NG-tagged ANME1G37 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pMRX-IP-3xFLAG-NCOA4 (dN)

    Plasmid

    #192808

    Purpose

    Stable expression of 3xFLAH-NCOA4 (delta N) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of the N-terminal domain

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMRX-IP-3xFLAG-NCOA4 (dM)

    Plasmid

    #192810

    Purpose

    Stable expression of 3xFLAH-NCOA4 (delta M) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of the Middle domain

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMRX-IP-3xFLAG-NCOA4 (dIDR1)

    Plasmid

    #192809

    Purpose

    Stable expression of 3xFLAH-NCOA4 (delta IDR1) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of the IDR1

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMRX-IP-3xFLAG-NCOA4 (dC)

    Plasmid

    #192812

    Purpose

    Stable expression of 3xFLAH-NCOA4 (delta C) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deletion of the C-terminal domain

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLentiGuide_mCherry_sgEPAS1#1

    Plasmid

    #183122

    Purpose

    pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgEPAS1-1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMRX-IP-3xFLAG-NCOA4 (I56E)

    Plasmid

    #192813

    Purpose

    Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    I56E mutation in the N-terminal domain

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMRX-IP-3xFLAG-NCOA4 (L63R)

    Plasmid

    #192814

    Purpose

    Stable expression of 3xFLAH-NCOA4 (L63R mutant) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

    Insert

    NCOA4 (NCOA4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    L63R mutation in the N-terminal domain

    Promoter

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. pLVX-RT006-GCN4-HaloPM

    Plasmid

    #163386

    Purpose

    To express 4X sGCN4 module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pWXLd-RIEP-CeNLuc

    Plasmid

    #135934

    Purpose

    Expresses the CeNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    CeNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. LbdCas12a KRAB

    Plasmid

    #188503

    Purpose

    Expresses FLAG tagged LbdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLZRS-IRES-deltaNGFR

    Plasmid

    #72930

    Purpose

    retroviral expression with FACS-selectable marker (extracellular part of NGFR)

    Depositor

    Esther van de Vosse

    Article

    van_de_Vosse et al Blood. 2009 May 7;113(19):4548-55. doi:

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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49. FLAG-TKGAPDH in pLJM60

    Plasmid

    #154217

    Purpose

    Lentiviral vector for stable expression of FLAG-TK-GAPDH

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    GAPDH
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    cDNA has been codon optimized for human cell expr…

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. p663-UBC-miniTurbo-V5-MARK1_IDG-K

    Plasmid

    #170582

    Purpose

    Gateway destination clone of MARK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-MARK1 (MARK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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