We narrowed to 4,854 results for: U6...

1. pAAV-ITR-EcYtR(NGS-6)-mCherry

   Plasmid

   #217363

   Purpose

   Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   E. coli tyrosine tRNA mutant, "NGS-6"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-ITR-PytR(PyOtR)-mCherry

   Plasmid

   #204870

   Purpose

   Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   M. mazei pyrrolysyl tRNA mutant "PyOtR"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

   Promoter

   U6

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cbh_v5 AAV-saCBE C-terminal

   Plasmid

   #137183

   Purpose

   AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-saCBE C-terminal
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. KAT2A sgRNA2

   Plasmid

   #138186

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human KAT2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   KAT2A (KAT2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. KAT2A sgRNA1

   Plasmid

   #138185

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human KAT2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   KAT2A (KAT2A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-sgLkb1/Cre

   Plasmid

   #66894

   Purpose

   Expresses an Lkb1-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

   Insert

   sgLkb1
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. WT1 KI gRNA2

   Plasmid

   #92313

   Purpose

   CRISPR-GFP-gRNA for cutting WT1

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Insert

   WT1 (WT1 Human)
   

   Use

   Tags

   CRISPR GFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCKOv3

   Plasmid

   #209046

   Purpose

   Lentiviral backbone for expressing U6 driven SpCas9 sgRNAs with BveI cloning sites, SpCas9 tracrRNAv1 (Dang et al., 2015; PMID: 26671237) and puromycin selection marker

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain

   Plasmid

   #187276

   Purpose

   Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domain

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   deltaActin binding domain

   Promoter

   U6, CMV

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTBL680 CHYRON3 integration construct

    Plasmid

    #126448

    Purpose

    To integrate the CHYRON3 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO_S.NWS.VSVg_NGFR

    Plasmid

    #158348

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.NWS.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

    Plasmid

    #187275

    Purpose

    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain

    Depositor

    Alpha Yap

    Article

    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

    Insert

    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    deltaMyosin binding domain

    Promoter

    U6, CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcBac1-Mb-Fluoro-Phe B5

    Plasmid

    #197576

    Purpose

    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

    Inserts

    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    Nuclear export sequence + FLAG

    Expression

    Mammalian

    Mutation

    N311G, C313A

    Promoter

    CMV and U6/H1

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

    Plasmid

    #176839

    Purpose

    To recruit SAM complex to human CYP3A4 proximal enhancer.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer sgRNA for activation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJW1838

    Plasmid

    #154324

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK1520-LRRK2-G2019S-ng

    Plasmid

    #180436

    Purpose

    Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-pegRNA-SNCA-A53T

    Plasmid

    #181738

    Purpose

    Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBPK1520-SNCA-A53T-ng

    Plasmid

    #181739

    Purpose

    Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing ngRNA for SNCA-A53T (SNCA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AOI-WT-Cas9-sg-mouse Suz12-F1-GFP

    Plasmid

    #91881

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Suz12 (Suz12 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pLKO_AU1.HSV.S_mCherry-NLS

    Plasmid

    #178224

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HSV.S and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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