We narrowed to 4,995 results for: mcs

1. pAAV-Syn-FLEX-ExSYTE

   Plasmid

   #216263

   Purpose

   Cre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

   Depositor

   Susumu Tomita

   Article

   Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

   Insert

   ExSYTE P2A EGFP - Rat DTE
   

   Use

   AAV

   Available Since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiEKAR2G2

   Plasmid

   #40178

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ1201

   Plasmid

   #44483

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, unc-119, peel-1, MCS
   

   Use

   Multiple cloning site vector

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2-nbt-Electra1-P2A-mScarlet

   Plasmid

   #196113

   Purpose

   zebrafish expression of nbt-Electra1-P2A-mScarlet

   Depositor

   Reinhard Köster

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   nbt-Electra1-P2A-mScarlet
   

   Use

   Zebrafish expression

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM124: pAAV-EFS-CasRx-VEGFA presgRNA

   Plasmid

   #166872

   Purpose

   AAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editing

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Inserts

   U6-VEGFA presgRNA
   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EFS and U6

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiEKAR2G1

   Plasmid

   #40177

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPReporter

   Plasmid

   #65007

   Purpose

   E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

   Depositor

   Mattheos Koffas

   Article

   Cress et al ACS Synth Biol. 2015 Mar 30.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   none

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-hCas9-T2A-mStrawberry

   Plasmid

   #187224

   Purpose

   Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Inserted T2A-mStrawberry at 3' terminal of M…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUW-TetO-DsRed-cNLS

   Plasmid

   #138533

   Purpose

   Lentiviral inducible expression of DsRed-Express2 with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   DsRed-Express2
   

   Use

   Lentiviral

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Promoter

   TRE-CMV

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. SEP-Nanoluc-cyOFP1

    Plasmid

    #141082

    Purpose

    prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEP-Nanoluc-cyOFP1
    

    Use

    Lentiviral

    Mutation

    Deletion of amino acids DISGG from positions 673 …

    Promoter

    EF1α

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-hCas9-T2A-miRFP670

    Plasmid

    #187223

    Purpose

    Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Cas9
    

    Tags

    Inserted T2A-miRFP670 at 3' terminal of MCS.

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAGGS-Flex-GFP

    Plasmid

    #197883

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFJ1202

    Plasmid

    #44484

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, peel-1, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH_ss-SBP-EGFP

    Plasmid

    #172358

    Purpose

    Reporter to monitoring a streptavidin-binding peptide trafficking

    Depositor

    Guido Kroemer

    Article

    Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

    Insert

    ER importing signal sequence
    

    Use

    Lentiviral

    Tags

    SBP-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQC hKGA.wt V5-His IRES G418

    Plasmid

    #110390

    Purpose

    γ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    KGA glutaminase (GLS Human)
    

    Use

    Retroviral

    Tags

    6xHis and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-NES-tdTomato-LINuS-WPRE

    Plasmid

    #159894

    Purpose

    AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

    Insert

    NES-tdTomato-LINuS
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1α promoter

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)

    Plasmid

    #52906

    Purpose

    Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[2]+Venus
    

    Use

    Retroviral

    Tags

    L-IFP-F[2]

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pAAVS1 Insulator ObLiGaRe/EPB71

    Plasmid

    #90018

    Purpose

    Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

    Depositor

    Eric-Paul Bennett

    Article

    Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

    Insert

    MCS flanked by insulator and inverted ZFN binding sites
    

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Hyg-FHIT

    Plasmid

    #16410

    Depositor

    Bert Vogelstein

    Article

    Kohli et al Nucleic Acids Res. 2004 . 32(1):e3.

    Insert

    FHIT Hyg targeting (FHIT Human)
    

    Use

    AAV; Adeno-associated viral

    Expression

    Mammalian

    Available Since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pIVEX2.3 TEM-5

    Plasmid

    #16561

    Depositor

    Bert Vogelstein

    Article

    Carson-Walter et al Cancer Res. 2001 Sep 15. 61(18):6649-55.

    Insert

    TEM5 (ADGRA2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Extracellular region

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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