We narrowed to 18,334 results for: met

1. pNHT7::YB1::sNluc5

   Plasmid

   #186762

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNHT7::RPL15::sNluc2_STOP

   Plasmid

   #186767

   Purpose

   An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Premature stop codon at residue 16

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNHT7::RPL15::sGluc

   Plasmid

   #186769

   Purpose

   An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Gaussia luciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pST_B4YSF1_9LAMI (pBS0347)

   Plasmid

   #185186

   Purpose

   For the mammalian expression of the Dorcoceras hygrometricum protein B4YSF1_9LAMI. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   B4YSF1_9LAMI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

   Plasmid

   #140455

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, leucine 624 to asparagine

   Promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

   Plasmid

   #140456

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, alanine 634 to asparagine

   Promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4_HLA-flag-GRM3 C127A-VN

   Plasmid

   #140449

   Purpose

   Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   FLAG tag, Signal/leader sequence from HLA class I…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine

   Promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4_HLA-myc-GRM3 C127A-VC

   Plasmid

   #140450

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine

   Promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

   Plasmid

   #140452

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, isoluecine 578 to alanine

   Promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid

    #140453

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, phenylalanine 588 to val…

    Promoter

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mYF

    Plasmid

    #171460

    Purpose

    Expresses mYF, a CiCE of NiCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    EYFP(A207K)-FKBP12
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mActZ-FmCh

    Plasmid

    #171465

    Purpose

    Expresses mActZ-FmCh, a FiCE of iCMM, in mammalian cells

    Depositor

    Takafumi Miyamoto

    Article

    Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

    Insert

    ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJC2-GPT1-3xFLAG

    Plasmid

    #163446

    Purpose

    lentiviral overexpression vector for human GPT1

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    GPT1 (GPT Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    CMV

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKM59

    Plasmid

    #154158

    Purpose

    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Enhancer for the Drosophila melanogaster Metchnik…

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY036_ATP1A1_G3

    Plasmid

    #86617

    Purpose

    Expresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2

    Plasmid

    #69228

    Purpose

    Expresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS2 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3

    Plasmid

    #69229

    Purpose

    Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4

    Plasmid

    #69230

    Purpose

    Expresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS4 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3

    Plasmid

    #69231

    Purpose

    Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 TALE
    

    Use

    CRISPR

    Tags

    2X NLS and NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. mIFP-C-Src-7

    Plasmid

    #56213

    Purpose

    Localization: Membrane/Vesicles, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    C-Src (SRC Chicken)
    

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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