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Showing: 4041 - 4060 of 25716 results
  1. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid
    #176149
    Purpose
    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette
    Depositor
    Insert
    X-ray repair cross complementing 1 (XRCC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…
    Promoter
    CMV
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3-eGFP-NLS-RNF168 delta RING

    Plasmid
    #133983
    Purpose
    mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)
    Depositor
    Insert
    RNF168 (RNF168 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    deletion of RING; siRNA resistant sequence: GAGGA…
    Promoter
    CMV
    Available since
    Nov. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. FRIG-myc-Sema4D-CD4

    Plasmid
    #51606
    Purpose
    expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone
    Depositor
    Insert
    Sema4D/CD4-FLAG (Sema4d Mouse, Human)
    Use
    Lentiviral
    Tags
    FLAG and myc
    Expression
    Mammalian
    Mutation
    Sema4D amino acids 660-861 were replaced with CD4…
    Promoter
    RSV
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  4. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pLenti KO CLDN1 2xgRNA - spCas9 iRFP670 puro

    Plasmid
    #166133
    Purpose
    This plasmid allows efficient KO of the cldn1 gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance
    Depositor
    Insert
    hU6-gRNA and hH1-gRNA targeting cldn1 gene
    Use
    CRISPR and Lentiviral
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

    Plasmid
    #166134
    Purpose
    This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance
    Depositor
    Insert
    hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
    Use
    CRISPR and Lentiviral
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 4041 - 4060 of 25716 results