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Bxb1-GA_AIO-TetOn_mScarlet_Divergent

Plasmid

#229791

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

Plasmid

#98927

Purpose

Cre dependent AAV expression of GFP spaghetti monster myc construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Has Service

AAV1

Insert

GFP-smFP-myc

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 Ub-S297

Plasmid

#169965

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Tags

Ub

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-ITPKA-3x-mScarlet-I

Plasmid

#229782

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

3x mScarlet-I

Use

Synthetic Biology

Tags

ITPKA

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shTREX1

Plasmid

#127700

Purpose

Doxycyclin inducible shRNA knockdown of both human and mouse TREX1 gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shTREX1 (Trex1 Mouse, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3C WT

Plasmid

#123095

Purpose

Expresses 3xFLAG-LC3C wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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σ1-mCherry

Plasmid

#186578

Purpose

Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP1S1 (AP1S1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB511

Plasmid

#74853

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-FOXF1

Plasmid

#153122

Purpose

Expression of Flag tagged human FOXF1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXF1 (FOXF1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L

Plasmid

#89576

Purpose

caCas9 integrating vector into the Neut5L locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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PTP1B human

Plasmid

#102719

Purpose

human PTP1B catalytic domain (residues 1-301) in RP1B vector

Depositor

Wolfgang Peti

Article

Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5.

Insert

Protein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB404

Plasmid

#74798

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-hFIP200

Plasmid

#38192

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

FIP200 (RB1CC1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
pGoldenGreenGate-M (pGGG-M)

Plasmid

#165422

Purpose

A Golden Gate (MoClo) compatible Level 2 acceptor binary vector based on pGreenII (Hellens et al., 2000).

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Level 2 golden gate (moclo) du…

Promoter

Contain GG cassette and MCS with LacZ for blue/wh…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP4

Plasmid

#42313

Depositor

Thierry Galli

Article

Mallard et al J Cell Biol. 2002 Feb 18;156(4):653-64.

Insert

VAMP 4 (VAMP4 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pTP2620

Plasmid

#113311

Purpose

human Rad50 in baculovirus transfer vector

Depositor

Tanya Paull

Article

Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

Insert

Rad50 (RAD50 Human)

Tags

HA, 6xhis

Expression

Insect

Promoter

polyhedrin

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG_mRuby2_smFP OLLAS

Plasmid

#59761

Purpose

encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_OLLAS

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98925

Purpose

AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

Ruby2-smFP-Flag

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19 PKC delta

Plasmid

#8427

Depositor

Frederic Mushinski

Article

Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

Insert

Mouse Protein Kinase C delta (Prkcd Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_Divergent Donor

Plasmid

#229784

Purpose

Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_Divergent Donor

Plasmid

#229786

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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LLP791_PB_dCas9-SSSavi_BirA

Plasmid

#211786

Purpose

PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

dCas9, Spy, Snoop, aGCN4, AviTags

Tags

3xHA

Expression

Mammalian

Mutation

dCas9 D10A and H840A

Promoter

pCAGG abd pEF1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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FlagMBNL1-41

Plasmid

#96906

Purpose

expresses Flag tagged human MBNL1-41 kDa isoform from pcDNA3.1 HisC

Depositor

Thomas Cooper

Article

Grammatikakis et al Nucleic Acids Res. 2011 Apr;39(7):2769-8

Insert

MBNL1-41 kDa isoform (MBNL1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6011nes

Plasmid

#108652

Purpose

An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-ERK-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB417

Plasmid

#74811

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-mScarlet

Plasmid

#211330

Purpose

Mammalian overexpression vector for C-terminally mScarlet tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1.1

Plasmid

#218822

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-FOXN1

Plasmid

#153137

Purpose

Expression of Flag tagged human FOXN1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXN1 (FOXN1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF2

Plasmid

#155347

Purpose

Express full-length human YTHDF2 with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF2 (YTHDF2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK-Zeo

Plasmid

#25736

Purpose

Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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