We narrowed to 9,202 results for: sacs

1. pET23PI-Sce ESARC D218A

   Plasmid

   #195608

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

   Depositor

   Frederick Gimble

   Article

   Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T928C, T929C, A942C

   Promoter

   T7

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET23PI-Sce ESARC

   Plasmid

   #195607

   Purpose

   Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

   Depositor

   Frederick Gimble

   Article

   Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

   Insert

   PI-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

   Promoter

   T7

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAT-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193314

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Use

   Tags

   4MYC

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HYG-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193316

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Use

   Tags

   4MYC

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SEC7-2Katushka-ter-Hygromycin

   Plasmid

   #184772

   Purpose

   Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   SEC7 (SEC7 Budding Yeast)
   

   Use

   Tags

   2Katushka

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PKC1-2GFP-ter-TRP1

   Plasmid

   #184773

   Purpose

   Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   PKC1 (PKC1 Budding Yeast)
   

   Use

   Tags

   2EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. OA-1127B

   Plasmid

   #190997

   Purpose

   Expresses arrays of gRNA targeting rel1 promoter under U6b/c promoter

   Depositor

   Omar Akbari

   Article

   Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

   Insert

   U6b:sgRNArel1A1 - U6c:sgRNArel1A2 - U6b:sgRNArel1A3 - U6c:sgRNArel1A4
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP4D42

   Plasmid

   #185840

   Purpose

   Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIALD2E

   Plasmid

   #185866

   Purpose

   Knocking out ALD6

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD(-125, 40)- ALD6(1054, 1749)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFP10CF71

    Plasmid

    #185860

    Purpose

    Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pILGFP10CF72 (B9)

    Plasmid

    #185858

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pILGFP10CF72

    Plasmid

    #185857

    Purpose

    Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pILGFP10E1B

    Plasmid

    #185856

    Purpose

    Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pILGFP10E1A

    Plasmid

    #185855

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pILGFP3A2 (1)

    Plasmid

    #185851

    Purpose

    Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAP01

    Plasmid

    #186260

    Purpose

    sfGFP under light light responsive PEL222 promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB9358(VII-1_Markerfree_BackBone)

    Plasmid

    #161599

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB9359(VIII-1_Markerfree_BackBone)

    Plasmid

    #161600

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfB9361(XIII-1_Markerfree_BackBone)

    Plasmid

    #161602

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB9363(XVI-1_Markerfree_BackBone)

    Plasmid

    #161604

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. KBB439

    Plasmid

    #185093

    Purpose

    NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    GAL1::NMA111-GFP

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. KBB462

    Plasmid

    #185095

    Purpose

    NMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    GAL1::nma111Dnls1Dnls2-GFP

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. KBB394

    Plasmid

    #185086

    Purpose

    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Nma111 KKR 9-11 AAA; KRK 28-30 AAA

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. KBB106

    Plasmid

    #185065

    Purpose

    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain

    Depositor

    Ken Belanger

    Article

    Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 C-terminal region, truncated so C-terminal h…

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTS2370-Tier1-PhCMV-TetR_F-type

    Plasmid

    #169573

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline-dependent F-type-based transactiavtor (PhCMV-TetR-F-type-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTS2373-Tier1-PhCMV-Gal4

    Plasmid

    #169581

    Purpose

    Tier-1 vector encoding PhCMV-driven Gal4 DNA binding domain (PhCMV-Gal4-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    regulatory protein GAL4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTriEx4_Rap1

    Plasmid

    #184662

    Purpose

    Mammalian expression of mouse Rap1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA-HA.HA.HA.Mm.RAP1

    Plasmid

    #184646

    Purpose

    Mammalian expression of mouse Rap1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    rap1
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CcCDP_p15A

    Plasmid

    #179272

    Purpose

    Expresses CcCDP in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Insert

    cellodextrin phosphorylase
    

    Use

    Synthetic Biology

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pnEA-NpM-Scp20_18-49-GB1_AF

    Plasmid

    #148720

    Purpose

    Bacterial Expression of Scp20_18-49

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

    Insert

    Scp20_18-49 (CAF20 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge